Bxy-fuca encoding α-L-fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus
Bxy-fuca encoding α-L-fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus
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编码 α-L-岩藻糖苷酶的 Bxy-fuca 在松材线虫松材线虫的发育和繁殖中起着至关重要的作用
DOI：
10.1002/ps.5497
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发表时间：
2020-01-01
影响因子：
4.1
通讯作者：
Zhang, Xingyao
中科院分区：
文献类型：
--
作者：
Tang, Jia;Ma, Ruoqing;Zhang, Xingyao
背景：松材线虫是引起松树枯萎病的病原。这种疾病对全球松树林构成严重威胁。fuca基因编码α-L-岩藻糖苷酶，其在细菌、真菌、植物和动物的许多生物和病理过程中起关键作用。为了找到有希望的针对PWD的控制策略，我们研究了Bxy-fuca在B中的表达和功能。结果：Bxy-fuca编码的α-L-岩藻糖苷酶在功能结构域和关键残基上高度保守。它在B的所有发育阶段持续表达。木生植物mRNA原位杂交结果表明，Bxy-fuca主要定位于体壁肌肉和肠道。RNA干扰表明Bxy-fuca的合子表达是胚胎发生所必需的。B的速率。Bxy-fuca干扰后，小菜蛾卵孵化率显著降低。Bxy-fuca的胚后沉默导致B的寿命和产生的卵的总数急剧下降。木生植物结论：Bxy-fuca对B的发育、寿命和繁殖具有重要作用。木生植物我们的研究结果为通过阻断胚胎发育和个体发育、降低线虫繁殖力以及破坏B之间的联系来控制PWD提供了有希望的线索。通过阻止线虫运动进入气管系统来控制松材线虫及其媒介甲虫。(c)2019化学工业协会
BACKGROUND: The pine wood nematode (PWN) Bursaphelenchus xylophilus is the causal agent of pine wilt disease (PWD). This disease is a serious threat to pine forests globally. The fuca gene encodes alpha-L-fucosidase, which plays crucial roles in numerous biological and pathological processes in bacteria, fungi, plants and animals. To find promising control strategies against PWD, we investigated the expression and functions of Bxy-fuca in B. xylophilus.RESULTS: Bxy-fuca encoding alpha-L-fucosidase is highly conserved within the deduced functional domains and key residues. It is expressed continuously across all developmental stages of B. xylophilus. mRNA in situ hybridization demonstrated that Bxy-fuca was mainly localized in the body wall muscles and intestine. RNA interference indicated that the zygotic expression of Bxy-fuca was indispensable for embryogenesis. The rate of B. xylophilus egg hatch was significantly decreased after Bxy-fuca was interfered. Postembryonic silence of Bxy-fuca resulted in a dramatic decrease in the longevity of and the total number of eggs produced by B. xylophilus. In addition, the motility of the nematode was greatly hampered with a significant drop in head thrashing frequency.CONCLUSION: Bxy-fuca plays crucial roles in development, lifespan and reproduction of B. xylophilus. Our results provide promising hints for control of PWD by blocking embryogenesis and ontogenesis, decreasing nematode fecundity, and disrupting the connection between B. xylophilus and its vector beetle by preventing nematode movement into the tracheal system. (c) 2019 Society of Chemical Industry