THE BONE MARROW-DERIVED STROMAL CELL-LINES MC3T3-G2/PA6 AND ST2 SUPPORT OSTEOCLAST-LIKE CELL-DIFFERENTIATION IN COCULTURES WITH MOUSE SPLEEN-CELLS
THE BONE MARROW-DERIVED STROMAL CELL-LINES MC3T3-G2/PA6 AND ST2 SUPPORT OSTEOCLAST-LIKE CELL-DIFFERENTIATION IN COCULTURES WITH MOUSE SPLEEN-CELLS
复制标题
DOI：
10.1210/endo-125-4-1805
复制
发表时间：
1989-10-01
期刊：
影响因子：
4.8
通讯作者：
SUDA, T
中科院分区：
文献类型：
--
作者：
UDAGAWA, N;TAKAHASHI, N;SUDA, T
在我们先前报道破骨细胞样多核细胞（MNC）响应于1 α而形成之后，25-二羟维生素D3 [1 α，25-（OH）2D 3]在小鼠脾细胞和从胎鼠颅骨新鲜分离的富含成骨细胞的群体的共培养物中，我们检查了在诱导破骨细胞样细胞形成中，这些原代成骨细胞样细胞是否可以被已建立的细胞系替代。我们首先使用两个克隆细胞系同时建立从新生小鼠颅骨。一个是成骨细胞系MC 3 T3-E1，另一个是前脂肪细胞系MC 3 T3-G2/PA 6。在1 α，25-（OH）2D3。地塞米松极大地增强了1 α诱导的TRACP阳性MNC形成，与此相反，成骨细胞MC 3 T3-E1在共培养中不能诱导TRACP阳性细胞。另一种骨髓来源的基质细胞系ST 2也在共培养物中诱导TRACP阳性MNC形成，以响应1 α，25-（OH）2D 3和地塞米松。鲑鱼降钙素只有在TRACP阳性细胞形成时才能增强共培养物中cAMP的产生。放射自显影研究表明，[25 I]降钙素特异性结合TRACP阳性细胞形成的共培养物。当脾细胞和MC 3 T3-G2/PA 6或ST 2细胞在1 α存在下在抹香鲸牙本质切片上共培养时，25-（OH）_2D_3和地塞米松作用后，形成大量吸收陷窝。这些结果表明，两种骨髓来源的基质细胞系可以支持破骨细胞样细胞分化为与脾细胞共培养。
After our previous report that osteoclast-like multinucleated cells (MNCs) were formed in response to 1.alpha.,25-dihydroxyvitamin D3 [1.alpha.,25-(OH)2D3] in cocultures of mouse spleen cells and osteoblast-rich populations freshly isolated from fetal mouse calvariae, we examined whether such primary osteoblast-like cells can be replaced by established cell lines in inducing osteoclast-like cell formation. We first used two clonal cell lines simultaneously established from newborn mouse calvariae. One was the osteoblastic cell line MC3T3-E1, and the other was the preadipose cell line MC3T3-G2/PA6. Tartrateresistant acid phosphatase (TRACP; a marker enzyme of osteoclasts)-positive mononuclear cells and MNCs were formed in the cocultures of spleen cells and MC3T3-G2/PA6 cells in the presence of 1.alpha.,25-(OH)2D3. Dexamethasone greatly potentiated TRACP-positive MNC formation induced by 1.alpha.,25-(OH)2D3, whereas the glucocorticoid alone had no effect on it. In contrast, osteoblastic MC3T3-E1 cells failed to induced TRACP-positive cells in the cocultures. Another bone marrow-derived stromal cell line ST2 also induced TRACP-positive MNC formation in the cocultures in response to 1.alpha.,25-(OH)2D3 and dexamethasone. Salmon calcitonin enhanced cAMP production in the cocultures only when TRACP-positive cells were formed. Autoradiographic studies demonstrated that [25I]calcitonin specifically bound to TRACP-positive cells formed in the cocultures. When spleen cells and either MC3T3-G2/PA6 or ST2 cells were cocultured on sperm whale dentine slices in the presence of 1.alpha.,25-(OH)2D3 and dexamethasone, numerous resorption lacunae were formed. These results show that the two bone marrow-derived stromal cell lines can support osteoclast-like cell differentiation to cocultures with spleen cells.