ANTIFREEZE PROTEIN PRODUCED ENDOGENOUSLY IN WINTER RYE LEAVES
ANTIFREEZE PROTEIN PRODUCED ENDOGENOUSLY IN WINTER RYE LEAVES
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DOI：
10.1104/pp.100.2.593
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发表时间：
1992-10-01
期刊：
影响因子：
7.4
通讯作者：
MOFFATT, BA
中科院分区：
文献类型：
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作者：
GRIFFITH, M;ALA, P;MOFFATT, BA
冷驯化后，冬黑麦（Secale cereale L.）能够承受在冰冻温度下细胞外冰的形成。我们现在表明，第一次，冷驯化的冬季黑麦植物含有内源性产生的抗冻蛋白。这种蛋白质是从冬黑麦叶子的质外体中提取的，在冷冻过程中，在那里形成了冰。经部分纯化后，该蛋白被鉴定为抗冻蛋白，因为它改变了冰晶的正常生长模式，并非依赖性地降低了水的冻结温度。
After cold acclimation, winter rye (Secale cereale L.) is able to withstand the formation of extracellular ice at freezing temperatures. We now show, for the first time, that cold-acclimated winter rye plants contain endogenously produced antifreeze protein. The protein was extracted from the apoplast of winter rye leaves, where ice forms during freezing. After partial purification, the protein was identified as antifreeze protein because it modified the normal growth pattern of ice crystals and depressed the freezing temperature of water noncolligatively.