Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins
Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins
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DOI：
10.1073/pnas.0406656102
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发表时间：
2005-01-18
影响因子：
11.1
通讯作者：
Knowles, DP
中科院分区：
文献类型：
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作者：
Brayton, KA;Kappmeyer, LS;Knowles, DP
边缘无浆立克次体是世界范围内最普遍的蜱传牲畜病原体，严重制约动物健康。A.边缘蜱在受感染的反刍动物中建立终身持久性，这些动物作为蜱获得和传播病原体的宿主。在哺乳动物宿主中，A. marginale通过改变由许多蛋白质组成的表面涂层产生抗原变体。通过对A.边缘，我们表明，这种表面涂层是由两个家庭占主导地位的免疫显性蛋白：MSP2超家族和MSP1超家族。在949个注释的编码序列中，只有62个被预测为外膜蛋白，其中49个属于这两个超家族之一。基因组包含不寻常的功能假基因，属于msp2超家族，并发挥不可或缺的作用，在表面涂层抗原变异，因此明显不同于假基因描述为副产品的还原进化在其他立克次体。
The rickettsia Anaplasma marginale is the most prevalent tick-borne livestock pathogen worldwide and is a severe constraint to animal health. A. marginale establishes lifelong persistence in infected ruminants and these animals serve as a reservoir for ticks to acquire and transmit the pathogen. Within the mammalian host, A. marginale generates antigenic variants by changing a surface coat composed of numerous proteins. By sequencing and annotating the complete 1,197,687-bp genome of the St. Maries strain of A. marginale, we show that this surface coat is dominated by two families containing immunodominant proteins: the msp2 superfamily and the msp1 superfamily. Of the 949 annotated coding sequences, just 62 are predicted to be outer membrane proteins, and of these, 49 belong to one of these two superfamilies. The genome contains unusual functional pseudogenes that belong to the msp2 superfamily and play an integral role in surface coat antigenic variation, and are thus distinctly different from pseudogenes described as byproducts of reductive evolution in other Rickettsiales.