Tandem chromosome fusions in karyotypic evolution of Muntiacus:: evidence from M-feae and M-gongshanensis
Tandem chromosome fusions in karyotypic evolution of Muntiacus:: evidence from M-feae and M-gongshanensis
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DOI：
10.1007/s10577-006-1073-2
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发表时间：
2006-09-01
影响因子：
2.6
通讯作者：
Yang, F.
中科院分区：
文献类型：
--
作者：
Huang, L.;Wang, J.;Yang, F.
猕猴桃（mununtiacus, Cervidae）以其通过重复的串联染色体融合而快速而彻底的核型多样化而闻名，构成了核型进化研究的典范。在已确定核型的5种猕猴中，目前已采用比较染色体显带、染色体绘制和BAC图谱相结合的方法对3种猕猴（Muntiacus reevesi, 2n = 46; m.m. vaginalis, 2n = 6/7; m.c riifrons, 2n = 8/9）进行了研究。结果表明，广泛的着丝粒-端粒融合和少量的中心融合是紫麂核型进化的染色体机制。在这里，我们将同样的方法应用于另外两个核型特征较差的麂种，M. feae （2n = 14雄性）和M. gongshanensis （2n = 8雌性）。提供了高分辨率的g带核型。通过对杂交结果的综合分析，建立了M. reevesi、M. feae和M. gongshanensis的高分辨率比较图谱，证明了支撑这两种麂核型进化的所有串联融合都是着丝粒-端粒融合。此外，研究结果提高了我们对现存麂种核型关系的认识，并提供了令人信服的细胞遗传学证据，支持M. crinifrons、M. feae和M. gongshanensis应被视为不同物种的观点。
The muntjacs (Muntiacus, Cervidae) are famous for their rapid and radical karyotypic diversification via repeated tandem chromosome fusions, constituting a paradigm for the studies of karyotypic evolution. Of the five muntjac species with defined karyotypes, three species (i.e. Muntiacus reevesi, 2n = 46; M. m. vaginalis, 2n = 6/7; and M. crinifrons, 2n = 8/9) have so far been investigated by a combined approach of comparative chromosome banding, chromosome painting and BAC mapping. The results demonstrated that extensive centromere-telomere fusions and a few centric fusions are the chromosomal mechanisms underlying the karyotypic evolution of muntjacs. Here we have applied the same approach to two additional muntjac species with less well-characterized karyotypes, M. feae (2n = 14 male) and M. gongshanensis (2n = 8 female). High-resolution G-banded karyotypes for M. feae and M. gongshanensis are provided. The integrated analysis of hybridization results led to the establishment of a high-resolution comparative map between M. reevesi, M. feae, and M. gongshanensis, proving that all tandem fusions underpinning the karyotypic evolution of these two muntjac species are also centromere-telomere fusions. Furthermore, the results have improved our understanding of the karyotypic relationships of extant muntjac species and provided compelling cytogenetic evidence that supports the view that M. crinifrons, M. feae, and M. gongshanensis should each be treated as a distinct species.