The Trypanosoma cruzi RNA-binding protein RBP42 is expressed in the cytoplasm throughout the life cycle of the parasite.
The Trypanosoma cruzi RNA-binding protein RBP42 is expressed in the cytoplasm throughout the life cycle of the parasite.
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克氏锥虫 RNA 结合蛋白 RBP42 在寄生虫的整个生命周期中在细胞质中表达。
DOI：
10.1007/s00436-018-5787-9
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发表时间：
2018
影响因子：
2
通讯作者：
Bellofatto,Vivian
中科院分区：
文献类型：
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作者：
TylerWeisbarth,R;Das,Anish;Castellano,Paul;Fisher,MichaelA;Wu,Han;Bellofatto,Vivian
克氏锥虫是引起人类恰加斯病的原生动物寄生虫，它有一个复杂的生命周期，可以促进在不同环境中的生存。在每种环境中，寄生虫都必须微调其代谢途径以进行分裂和繁殖。在缺乏可识别的转录基因调控的情况下，很明显，蛋白质水平是由转录后机制决定的。转录后基因控制受靶向细胞质中mRNA的RNA结合蛋白的影响。为了启动克氏锥虫的转录后活性研究，我们研究了这种生物体的RBP42（锥虫RNA结合蛋白）的直系同源物。RBP42最初是在布氏锥虫中检测到的，并显示其靶向编码控制中心碳代谢的蛋白质的mRNA的子集。cruziRBP42在结构上是不规则的。布鲁氏菌RBP42，在其氨基末端共享NTF2结构域，在其羧基末端共享单个RNA结合结构域（具体地，RNA识别基序，或RRM）。系统发育分析表明，NTF2和单一RRM是广泛动质体组内所有RBP42直系同源物的区别特征。cruziRBP42在寄生虫的所有生命周期阶段中表达，如通过免疫印迹和免疫荧光显微镜所确定的。在每种情况下，蛋白质都定位于细胞质，表明T的作用。cruziRBP42在寄生虫生命周期的所有阶段的转录后活性。我们推测RBP42影响负责寄生虫感染和传播的动态代谢途径。
Trypanosoma cruzi, the protozoan parasite that causes Chagas disease in humans, has a complex life cycle that promotes survival in disparate environments. In each environment, the parasite must fine-tune its metabolic pathways to divide and multiply. In the absence of recognizable transcriptional gene regulation, it is apparent that protein levels are determined by post-transcriptional mechanisms. Post-transcriptional gene control is influenced by RNA-binding proteins that target mRNAs in the cell’s cytoplasm. To initiate the study of post-transcriptional activities inT. cruzi, we studied this organism’s ortholog of RBP42, a trypanosomal RNA-binding protein. RBP42 was originally detected inTrypanosoma bruceiand was shown to target a subset of mRNAs that encode proteins governing central carbon metabolism.T. cruziRBP42 structurally resemblesT. bruceiRBP42, sharing an NTF2 domain at its amino terminus and a single RNA-binding domain (specifically, the RNA recognition motif, or RRM), at its carboxy terminus. A phylogenetic analysis reveals that an NTF2 and a single RRM are distinguishing features of all RBP42 orthologs within the broad kinetoplastid grouping.T. cruziRBP42 is expressed in all life cycle stages of the parasite as determined by immunoblot and immunofluorescence microscopy. In each case, the protein is localized to the cytoplasm, indicating a role forT. cruziRBP42 in post-transcriptional activities in all stages of the parasite life cycle. We speculate that RBP42 influences the dynamic metabolic pathways responsible for parasite infection and transmission.