Identification of HIV‐1 in semen following primary HIV‐1 infection
Identification of HIV‐1 in semen following primary HIV‐1 infection
复制标题
原发性 HIV-1 感染后精液中 HIV-1 的鉴定
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
D. Cooper
中科院分区：
文献类型：
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作者：
B. Tindall;L. Evans;P. Cunningham;P. McQueen;L. Hurren;E. Vasak;J. Mooney;D. Cooper
目的确定在感染后的最初几周内是否可以在精液样本中鉴定出HIV。设计一系列的三个同性恋男子有症状的原发性HIV-1感染。方法采用病毒培养、聚合酶链反应（PCR）和透射电镜技术对精液进行HIV-1检测。结果每例受试者的第一次样本（发病后17、22和24天）PCR均为阳性，病毒培养均为阴性。在原发性HIV-1感染后的前80天内获得的13个样本中，10个样本呈阳性。这些样品中只有一个是病毒培养阳性。在齐多夫定治疗期间从两名受试者中获得的四份精液样本PCR阳性。通过电子显微镜检查8个样本是否存在HIV-1，发现1个样本呈阳性。结论男性HIV-1感染者在感染后的最初几周内可能通过性传播感染。研究结果强调，处于HIV-1感染各个阶段的个人应采取更安全的性行为，以减少HIV-1的传播。
ObjectiveTo determine whether HIV could be identified in semen samples during the first few weeks after infection. DesignA series of three homosexual men with symptomatic primary HIV-1 infection. MethodsEach subject provided a series of semen samples that was examined for HIV-1 by virus culture, polymerase chain reaction (PCR) and transmission electron micrography. ResultsThe first samples obtained for each subject (17, 22 and 24 days following onset of primary HIV-1 infection) were all positive by PCR and negative by viral culture. Of 13 samples obtained during the first 80 days after onset of primary HIV-1 infection and analysed by PCR, 10 were positive. Only one of these samples was virus culture-positive. Four semen samples obtained from two subjects during treatment with zidovudine were PCR-positive. Eight samples were examined for presence of HIV-1 by electron microscopy and one was found to be positive. ConclusionsThese results indicate that men with HIV-1 infection are potentially infectious through sexual transmission during the first few weeks after infection. The findings emphasize that individuals in all stages of HIV-1 infection should practise safer sex to reduce transmission of HIV-1.