Impact of Porosity and Electrolyte Composition on the Surface Charge of Hydroxyapatite Biomaterials
Impact of Porosity and Electrolyte Composition on the Surface Charge of Hydroxyapatite Biomaterials
复制标题
DOI：
10.1021/acsami.5b10404
复制
发表时间：
2016-01-13
影响因子：
9.5
通讯作者：
Ginebra, Maria-Pau
中科院分区：
文献类型：
--
作者：
Espanol, Montserrat;Mestres, Gemma;Ginebra, Maria-Pau
材料植入体内的成败在很大程度上取决于材料的表面性质和宿主组织的反应。植入后发生的第一个事件是生物环境中的可溶离子、分子和蛋白质与材料表面的相互作用，导致形成吸附的蛋白质层，随后影响细胞附着。在这种情况下，材料的特殊形貌和表面电荷变得至关重要，因为它们影响将吸附的蛋白质的性质。然而，有关多孔衬底表面电荷的信息非常有限。直到最近，才准确地测定了多孔膜上的Zeta电位。这项工作的目标是实施之前的发现，以确定一系列多孔羟基磷灰石(HA)的Zeta电位，并评估孔隙率如何影响测量。此外，还进行了使用不同电解质的研究，以证明某些离子对HA的特定亲和力如何进一步影响表面电荷。结果表明，所有材料都表现出非常相似的外表面电荷(约-23 mV)，这与它们几乎相同的形貌一致。然而，样品表面下相互连接的气孔的存在导致了额外的内部Zeta电位，该电位随着孔隙率含量的变化而变化。用不同的电解质进行的测量证实了二价离子对HA的选择性，这表明使用相关的电解质测试生物材料的重要性。
The success or failure of a material when implanted in the body is greatly determined by the surface properties of the material and the host tissue reactions. The very first event that takes place after implantation is the interaction of soluble ions, molecules and proteins from the biological environment with the material surface leading to the formation of an adsorbed protein layer that will later influence cell attachment. In this context, the particular topography and surface charge of a material become critical as they influence the nature of the proteins that will adsorb. However, very limited information is available on the surface charge of porous substrates. Only until very recently was the determination of the zeta potential on porous membranes accurately determined. The goal of this work was to implement the previous findings for the determination of the zeta potential of a series of porous hydroxyapatite (HA) substrates and to assess how porosity affects the measurements. In addition, studies using various electrolytes were also performed to prove how the specific affinity of certain ions for HA can further impact surface charge. The results showed that all materials exhibited very similar external surface charge (approximately -23 mV), consistent with their almost identical topographies. However, the presence of interconnected pores underneath the sample surface resulted in an additional internal zeta potential that varied with the porosity content. Measurements with different electrolytes confirmed the selectivity of divalent ions for HA underlying the importance of testing biomaterials using relevant electrolytes.