IDENTIFICATION OF OSTEOCLAST PRECURSORS IN MULTILINEAGE HEMATOPOIETIC COLONIES
IDENTIFICATION OF OSTEOCLAST PRECURSORS IN MULTILINEAGE HEMATOPOIETIC COLONIES
复制标题
DOI：
10.1210/endo-128-1-259
复制
发表时间：
1991-01-01
期刊：
影响因子：
4.8
通讯作者：
CHAMBERS, TJ
中科院分区：
文献类型：
--
作者：
HATTERSLEY, G;KERBY, JA;CHAMBERS, TJ
已知破骨细胞来源于造血干细胞，但其谱系和调节其分化的机制在很大程度上是未知的。 有证据表明，骨髓基质细胞和造血前体细胞之间的接触依赖性相互作用诱导成骨细胞分化。 为了分析成骨细胞谱系，在促红细胞生成素与Wehi 3B条件培养基或白细胞介素3（IL 3）存在下，在半固体培养基中从小鼠脾细胞产生集落。 7天后，挑取单个菌落。 通过细胞离心涂片制备物中的细胞形态对每个集落的一半进行表型分析;将每个集落的另一半与骨髓来源的细胞系（ts 8）一起孵育7天，该细胞系在1，25-二羟基维生素D3存在下在骨切片上诱导造血细胞向骨细胞分化。 孵育后，通过扫描电子显微镜评估骨吸收。 没有再吸收诱导来自单系集落的细胞，但分化的粒细胞-巨噬细胞（GM）集落和38%的多系集落的再吸收细胞。 由于只有少数GM集落含有破骨细胞前体，这表明含有破骨细胞的GM集落不是典型的GM集落，但可能是一种多谱系集落，类似于含有粒细胞、巨噬细胞和第三种细胞类型的其他多谱系集落。 当IL 3衍生的集落在没有ts 8细胞的骨切片上孵育时，没有形成吸收细胞。 结果表明，破骨细胞来源于多系前体，IL 3作用于该前体以产生能够成骨细胞分化的细胞，该细胞在1，25-二羟维生素D3存在下与骨髓基质细胞孵育后形成吸收细胞。
The osteoclast is known to be derived from the hemopoietic stem cell, but its lineage and the mechanisms by which its differentiation is regulated are largely unknown. There is evidence that osteoclastic differentiation is induced through a contact-dependent interaction between bone marrow stromal cells and hemopoietic precursors. To analyze osteoclastic lineage, colonies were generated in semi-solid medium from mouse spleen cells in the presence of erythropoietin with either Wehi 3B-conditioned medium or interleukin 3 (IL3). After 7 days, individual colonies were picked. Half of each colony was phenotyped by the morphology of cells in cytospin preparations; the second half of each was incubated for 7 days with a bone marrow-derived cell line (ts8) that induces osteoclastic differentiation from hemopoietic cells, on bone slices in the presence of 1,25-dihydroxyvitamin D3. After incubation, bone resorption was assessed by scanning electron microscopy. No resorption was induced in cells derived from single-lineage colonies, but resorptive cells differentiated in 17% of granulocyte-macrophage (GM) colonies and 38% of multilineage colonies. Since only a minority of GM colonies contained osteoclastic precursors, this suggests that the GM colonies that contained osteoclasts were not typical GM colonies but may have been a form of multilineage colony analagous to other multilineage colonies that contain granulocytes, macrophages, and a third cell type. No resorptive cells were formed when IL3-derived colonies were incubated on bone slices without ts8 cells. The results suggest that osteoclasts are derived from a multilineage precursor, upon which IL3 acts to generate cells capable of osteoclastic differentiation, which form resorptive cells upon incubation with bone marrow stromal cells in the presence of 1,25-dihydroxyvitamin D3.