A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export
A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export
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DOI：
10.1073/pnas.95.13.7427
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发表时间：
1998-06-23
影响因子：
11.1
通讯作者：
Silver, PA
中科院分区：
文献类型：
--
作者：
Taura, T;Krebber, H;Silver, PA
在YRB 2中突变的酵母细胞（其编码与其他Ran结合蛋白相似的核蛋白）不能将包含核输出信号（内斯）的蛋白质（包括HIV Rev）输出到细胞核外。与内斯受体Xpo 1 p/Crm 1 p/exportin不同，Yrb 2 p不会在细胞核和细胞质之间穿梭，而是保留在细胞核内。然而，根据生化和遗传标准，Yrb 2 p与Xpo 1 p相互作用，而不与importin/karyopherin β超家族的其他成员相互作用。此外，含有核孔蛋白样FG重复的Yrb 2 p区域对于NES介导的蛋白输出是重要的。综上所述，这些数据表明，Yrb 2 p在细胞核内起作用，介导Xpo 1 p在几种核输出途径中的至少一种中的作用。
Yeast cells mutated in YRB2, which encodes a nuclear protein with similarity to other Ran-binding proteins, fail to export nuclear export signal (NES)-containing proteins including HIV Rev out of the nucleus. Unlike Xpo1p/ Crm1p/exportin, an NES receptor, Yrb2p does not shuttle between the nucleus and the cytoplasm but instead remains inside the nucleus. However, by both biochemical and genetic criteria, Yrb2p interacts with Xpo1p and not with other members of the importin/karyopherin beta superfamily. Moreover, the Yrb2p region containing nucleoporin-like FG repeats is important for NES-mediated protein export. Taken together, these data suggest that Yrb2p acts inside the nucleus to mediate the action of Xpo1p in at least one of several nuclear export pathways.