Up-regulation of heat shock proteins is essentail for cold survival during insect diapause
Up-regulation of heat shock proteins is essentail for cold survival during insect diapause
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DOI：
10.1073/pnas.0703538104
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发表时间：
2007-07-03
影响因子：
11.1
通讯作者：
Denlinger, David L.
中科院分区：
文献类型：
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作者：
Rinehart, Joseph P.;Li, Aiqing;Denlinger, David L.
滞育是越冬昆虫常见的休眠状态，它引发了一种独特的基因表达模式。在果蝇体内，大多数(但不是全部)苍蝇的热休克蛋白(HSP)表达上调。滞育上调的HSP包括HSP70家族的两个成员、HSP60家族的一个成员(TCP-1)、至少四个小的HSP家族成员和一个小的HSP假基因。Hsp70同源基因Hsc70的表达不受滞育的影响，而Hsp90实际上在滞育期间下调，因此滞育不同于引起所有HSPs同步上调的常见应激反应。热休克蛋白的上调开始于滞育开始，持续整个越冬期，并在苍蝇收到重新启动发育的信号后几个小时内停止。HSPs的上调在包括双翅目、鳞翅目、鞘翅目和膜翅目在内的不同昆虫目的滞育物种中以及在不同发育阶段(胚胎、幼虫、蛹、成虫)的滞育中都是常见的。利用RNAi技术抑制果蝇体内Hsp23和Hsp7O的表达，并未改变果蝇进入滞育的决定或滞育的持续时间，但对家蝇的低温生存能力有深远的影响。因此，我们认为，滞育期间HSPs的上调是越冬昆虫抗寒性的一个主要因素。
Diapause, the dormancy common to overwintering insects, evokes a unique pattern of gene expression. in the flesh fly, most, but not all, of the fly's heat shock proteins (Hsps) are up-regulated. The diapause up-regulated Hsps include two members of the Hsp70 family, one member of the Hsp60 family (TCP-1), at least four members of the small Hsp family, and a small Hsp pseudogene. Expression of an Hsp70 cognate, Hsc70, is uninfluenced by diapause, and Hsp90 is actually down-regulated during diapause, thus diapause differs from common stress responses that elicit synchronous up-regulation of all Hsps. Up-regulation of the Hsps begins at the onset of diapause, persists throughout the overwintering period, and ceases within hours after the fly receives the signal to reinitiate development. The up-regulation of Hsps appears to be common to diapause in species representing diverse insect orders including Diptera, Lepidoptera, Coleoptera, and Hymenoptera as well as in diapauses that occur in different developmental stages (embryo, larva, pupa, adult). Suppressing expression of Hsp23 and Hsp7O in flies by using RNAi did not alter the decision to enter diapause or the duration of diapause, but it had a profound effect on the pupa's ability to survive low temperatures. We thus propose that up-regulation of Hsps during diapause is a major factor contributing to cold-hardiness of overwintering insects.