The nociceptive mechanism of 5-hydroxytryptamine released into the peripheral tissue in acute inflammatory pain in rats
The nociceptive mechanism of 5-hydroxytryptamine released into the peripheral tissue in acute inflammatory pain in rats
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DOI：
10.1016/j.ejpain.2008.06.007
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发表时间：
2009-05-01
影响因子：
3.6
通讯作者：
Saito, Shigeru
中科院分区：
文献类型：
--
作者：
Nakajima, Kunie;Obata, Hideaki;Saito, Shigeru
本研究探讨了5-羟色胺（5-HT）在大鼠急性外周炎性痛中的作用。本研究采用福尔马林试验。福尔马林注射到大鼠后爪后，观察到双相疼痛相关行为（阶段I和2）。微透析研究表明，5-HT以福尔马林浓度依赖性方式（1.25-5%）释放到福尔马林注射部位，其峰值时间为注射后18 min。以往的研究表明，外周5-HT 2受体参与炎症性疼痛。因此。我们接下来检查了5-HT 2A和5-HT 2C受体是否参与，以及在福尔马林试验中5-HT从何处释放。用选择性5-HT 2A受体拮抗剂酮色林和选择性5-HT 2C受体拮抗剂RS 102221和SB 242084局部预处理以剂量依赖性方式抑制福尔马林试验第2阶段（第2A阶段）早期的退缩次数。用色甘酸钠（cromoglycate，croplyn）进行外周预处理。肥大细胞膜稳定剂，完全抑制5-HT释放，抑制福尔马林试验的2相反应。这些药物在福尔马林注射后2小时抑制了L4-5节段脊髓背角浅层c-fos的表达。这些结果表明，5-HT释放到外周组织及其受体，5-HT 2A和5-HT 2C，在急性外周炎症过程中的疼痛相关行为中起重要作用。(C)2008年，国际疼痛研究协会欧洲分会联合会。由Elsevier Ltd.出版，保留所有权利。
The present study examined the contribution of 5-hydroxytryptamine (5-HT) to acute peripheral inflammatory pain in rats. We used formalin test in this Study. After formalin injection into the rat hind paw, biphasic pain-related behavior (phases I and 2) was observed. A microdialysis study revealed that 5-HT was released into the formalin injection site in a formalin concentration-dependent manner (1.25-5%), and its peak time was 18 min after the injection. Previous studies suggest that peripheral 5-HT2 receptors are involved in inflammatory pain. Therefore. we next examined whether 5-HT2A and 5-HT2C receptors are involved, and from where 5-HT is released in the formalin test. Local pretreatment with a selective 5-HT2A receptor antagonist, ketanserin, and selective 5-HT2C receptor antagonists, RS102221 and SB242084, inhibited the number of flinches in early part of phase 2 (phase 2A) of the formalin test in a dose-dependent manner. peripheral pretreatment with sodium cromoglycate (cromolyn). a mast cell membrane stabilizer, completely Suppressed 5-HT release and inhibited phase 2 responses of the formalin test. These drugs inhibited c-fos expression in the superficial layer of the spinal dorsal horn of segments L4-5 at 2 It after formalin injection. These results indicate that 5-HT released into peripheral tissue and its receptors, 5-HT2A as well as 5-HT2C, at the periphery have an important role in pain-related behaviors during acute peripheral inflammation. (C) 2008 European Federation of Chapters of the International Association for the Study of Pain. published by Elsevier Ltd. All rights reserved.