Influence of domestication process on immune response to repeated emersion stressors in Eurasian perch (Perca fluviatilis, L.)
Influence of domestication process on immune response to repeated emersion stressors in Eurasian perch (Perca fluviatilis, L.)
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DOI：
10.1016/j.cbpa.2014.03.012
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发表时间：
2014-07-01
影响因子：
2.3
通讯作者：
Kestemont, P.
中科院分区：
文献类型：
--
作者：
Douxfils, J.;Lambert, S.;Kestemont, P.
驯化可能是一种可能的方式，以减少长期的应激源的生理反应和对免疫的有害影响。本研究的目的是评估慢性免疫反应诱导的反复浮现和驯化的可能影响，通过比较养殖的欧亚鲈鱼与短期（F1）和长期（F4）的圈养生活史。在第一个实验中，鱼暴露于一个单一的再现和生理应激反应，在短期内测量，以表征鱼的敏感性测试的压力。应激后6 h内血清皮质醇和葡萄糖升高，48 h内脾指数下降，表明该物种受到了再现应激的影响。在第二个实验中，F1和F4代进行重复的水浸提（在44天内每周3次）。在第9、18和44天，在应激后48小时进行取样以突出免疫系统的任何持续破坏。血清皮质醇，葡萄糖，SSI和溶菌酶活性进行了评价和血清蛋白质组分析，使用2D-DIGE。任何测试的变量都受到重复再现的影响，并且蛋白质组学分析仅揭示了α-2巨球蛋白（α 2 Ms）在应激个体的血清中上调。驯化还导致五种α 2 M同种型的上调和补体C3和IG轻链蛋白的下调，与任何应激物暴露无关。总之，研究结果表明，反复出现的欧亚鲈鱼并不严重的压力，可能解释了为什么驯化没有影响鱼类的反应。与驯化相关的变化是非常复杂的，当然需要进一步的调查。(C)2014 Elsevier Inc. All rights reserved.
Domestication might be a possible way to reduce the physiological response to long-term stressors and deleterious effects on immunity. The present study aimed to evaluate the chronic immune response induced by repeated emersions and the possible impact of domestication by comparing farmed Eurasian perch with short (F1) and long (F4) captive-life history. In the first experiment, fish were exposed to a single emersion and physiological stress response was measured in the short term to characterize fish sensitivity to the tested stressor. Serum cortisol and glucose elevated within 6 h post-stress and splenosomatic index (SSI) decreased within 48 h, indicating that the species was affected by emersion stressor. In the second experiment, F1 and F4 generations were submitted to repeated water emersions (3 times/week during 44 days). On day 9, 18 and 44, samplings were performed 48 h post-stressor to highlight any sustained disruption of immune system. Serum cortisol, glucose, SSI and lysozyme activity were evaluated and serum proteome was analyzed using 2D-DIGE. Any of the tested variables were affected by repeated emersions and proteomic analysis only revealed that alpha-2 macroglobulins (a2Ms) were up-regulated in the serum of stressed individuals. Domestication also resulted in the up-regulation of five a2M isoforms and down-regulation of complement C3 and Ig light chain proteins, independently of any stressor exposure. In conclusion, the results suggested that repeated emersions are not severe stressors for Eurasian perch, probably explaining why domestication had no influence on fish responses. Changes associated with domestication are highly complex and certainly need further investigations. (C) 2014 Elsevier Inc. All rights reserved.