Transferrin receptor in proliferation of T lymphocytes in infants with iron deficiency
Transferrin receptor in proliferation of T lymphocytes in infants with iron deficiency
复制标题
DOI：
10.1111/j.1365-2257.2006.00848.x
复制
发表时间：
2007-08-01
影响因子：
3
通讯作者：
Yesilipek, A.
中科院分区：
文献类型：
--
作者：
Artac, H.;Coskun, M.;Yesilipek, A.
本研究的目的是为了阐明缺铁时细胞免疫功能低下的机制。我们研究了缺铁(伴和不伴贫血)婴儿T淋巴细胞表面转铁蛋白受体(TFR)CD71的表达。共有33名年龄在7到26个月之间的婴儿参加了这项研究。这些受试者被分成三组：(I)隐性缺铁(LID)(组1)，(Ii)缺铁性贫血(IDA)(组2)，(Iii)健康婴儿(组3)。用流式细胞仪检测植物血凝素(PHA)刺激前后T淋巴细胞CD3和CD71的表达水平。经PHA刺激后，缺铁性贫血患儿外周血中CD3(+)淋巴细胞百分率明显低于对照组(48.6±10.5%vs.70.7+/-7.8%，P<0.001)。经PHA刺激后，三组T淋巴细胞(CD3+CD71%)转铁蛋白受体表达均升高(P<0.001)。CD3+CD71%三组间差异无统计学意义。尽管IDA患儿T淋巴细胞增殖能力下降，但T淋巴细胞表面转铁蛋白受体的表达能力正常。
The aim of this study was to contribute to clarify the mechanism of cellular immune insufficiency occurring during iron deficiency. We studied the expression of the transferrin receptor (TfR) which is called as CD71, on the surface of T lymphocytes in infants with iron deficiency (with and without anemia). A total of 33 infants, aged between 7 and 26 months were included in this study. These subjects were divided into three groups: (i) latent iron deficiency (LID) (group 1), (ii) iron deficiency anemia (IDA) (group 2), and (iii) healthy infants (group 3). Both CD3 levels and CD71 expression of T lymphocytes were analysed by flow cytometry before and after phytohaemagglutinin (PHA) stimulation. The percentage of CD3(+) lymphocytes in infants with IDA was lower than that in controls after PHA stimulation (mean +/- SD, 48.6 +/- 10.5%vs. 70.7 +/- 7.8%, P < 0.001). The TfR expression of T lymphocytes (CD3 + CD71%) increased in all three groups after PHA stimulation (P < 0.001). No significant difference was seen among the three groups with respect to CD3 + CD71%. Although there was a reduction in the proliferative capacity of T lymphocytes in infants with IDA, their ability to express transferrin receptor on T-lymphocyte cell surface was normal.