Role of Postnatal Expression of Fgfr1 and Fgfr2 in Testicular Germ Cells on Spermatogenesis and Fertility in Mice
Role of Postnatal Expression of Fgfr1 and Fgfr2 in Testicular Germ Cells on Spermatogenesis and Fertility in Mice
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发表时间：
2014-07
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通讯作者：
Shengqiang Li;Zi-Jian Lan;Xian Li;Jing Lin;Z. Lei
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作者：
Shengqiang Li;Zi-Jian Lan;Xian Li;Jing Lin;Z. Lei
背景 成纤维细胞生长因子 (FGF) 信号传导被认为在男性生殖系统中发挥着多种作用。然而，它在睾丸细胞精子发生和生育能力中的作用仍不清楚。方法本研究通过RT-PCR、Western blotting和免疫组化检测Fgfr 1（FGF受体）和Fgfr 2在出生后小鼠睾丸中的表达和定位。使用生殖细胞特异性 Fgfr 突变动物、Tex101-iCre;Fgfr flox/flox 和 Tex101-iCre;Fgfr flox/flox 小鼠测定睾丸生殖细胞中每种受体的体内功能。通过Kruskal-Wallis检验和Dunn后检验对结果进行分析。结果 Fgfr1和Fgfr2在整个出生后发育过程中均在睾丸中表达。在间质和管周细胞中观察到这些 FGFR 的显着免疫染色，在出生后发育的所有阶段几乎没有或没有变化。在包括细长精子细胞和精子在内的生殖细胞中也检测到这些受体的阳性染色。生殖细胞特异性 Fgfr1 或 Fgfr2 突变小鼠能够存活，睾丸和副性器官没有发育异常。生育力研究表明，两种突变小鼠品系的生育力与野生型同胞没有显着差异（n=4，p>0.05）。进一步分析表明，睾丸生殖细胞中还存在其他 Fgfr，包括 Fgfr 3、4 和 5。 结论 结果表明，Fgfr1 和 2 在所有睾丸细胞类型中表达，并且睾丸生殖细胞中的 Fgfr1 和 Fgfr2 对于精子发生和生育能力都不是必需的。未来的研究需要调查男性生殖细胞中五个 Fgfr 在精子发生和生育能力方面的潜在功能冗余。
Background Fibroblast growth factor (FGF) signaling is thought to play diverse roles in the male reproductive system. However, its role in testicular cells for spermatogenesis and fertility remains unclear. Methods In this study, the expression and localization of Fgfr 1 (FGF Receptor) and Fgfr 2 in the postnatal mouse testes were examined by RT-PCR, Western blotting and immunohistochemistry. The in vivo function of each receptor in testicular germ cells was determined using germ cell-specific Fgfr mutant animals, Tex101-iCre;Fgfr flox/flox and Tex101-iCre;Fgfr flox/flox mice. The results were analyzed by Kruskal-Wallis test and Dunn's Post-test. Results Both Fgfr1 and Fgfr2 were expressed in the testis throughout the entire postnatal development. Prominent immunostaining of these FGFRs was observed in interstitial and peritubular cells with little or no changes in all phases during postnatal development. Positive staining of these receptors was also detected in germ cells including elongated spermatids and spermatozoa. Germ cell-specific Fgfr1 or Fgfr2 mutant mice were viable with no developmental abnormalities in the testes and accessory sex organs. Fertility studies showed that the fecundity of both mutant mouse lines did not significantly differ from wild-type siblings (n=4, p>0.05). Further analysis indicated the presence of other Fgfrs in testicular germ cells including Fgfr 3, 4 and 5. Conclusion The results demonstrated that Fgfr1 and 2 are expressed in all testicular cell types and that neither Fgfr1 nor Fgfr2 in testicular germ cells is essential for spermatogenesis and fertility. Future studies are needed to investigate the potential functional redundancy among five Fgfrs in male germ cells for spermatogenesis and fertility.