HTLV-III expression in infected lymph nodes and relevance to pathogenesis of lymphadenopathy.
HTLV-III expression in infected lymph nodes and relevance to pathogenesis of lymphadenopathy.
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HTLV-III 在感染淋巴结中的表达及其与淋巴结病发病机制的相关性。
DOI：
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发表时间：
1986
影响因子：
6
通讯作者：
M. Harper
中科院分区：
文献类型：
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作者：
P. Biberfeld;K. J. Chayt;L. Marselle;G. Biberfeld;R. Gallo;M. Harper
用35 S标记的探针从14个淋巴结的HTLV-III/LAV-血清阳性的个人与淋巴结病的冷冻切片原位杂交显示存在罕见的细胞含有HTLV-III RNA的水平与病毒复制一致。观察到阳性细胞的频率仅为1-10个/切片。在研究的所有淋巴结中，包括显示滤泡增生或退化的淋巴结，大多数表达病毒RNA的细胞存在于滤泡区域。只有少数这样的细胞被发现以外的卵泡。从处理的冷冻切片中，滤泡病毒复制细胞的性质不明显。长时间放射自显影后，观察到另一种杂交模式，表明低水平的病毒RNA弥漫分布在大多数，但不是所有的卵泡。这可能代表细胞外病毒和/或病毒-抗体复合物，但与细胞内病毒RNA或吞噬的病毒颗粒不一致。与组织学相关的杂交水平的比较表明，随着滤泡退化的进展，HTLV-III复制细胞的数量以及弥散分布的病毒RNA的数量都有所减少。我们的观察结果表明，在HTLV-III感染的受试者，淋巴结滤泡是病毒复制和捕获的重要网站，这表明了决定性的作用，该病毒在HTLV-III相关的淋巴结病的特征性反应和致细胞病变的变化。
In situ hybridization with a 35S-labeled probe of cryosections from 14 lymph nodes of HTLV-III/LAV-seropositive individuals with lymphadenopathy showed the presence of rare cells containing HTLV-III RNA at levels consistent with virus replication. Positive cells were observed at a frequency of only 1-10 per section. In all lymph nodes studied, including those showing follicular hyperplasia or involution, most of the cells expressing viral RNA were present in the follicular areas. Only a minority of such cells were found outside the follicles. The nature of the follicular, virus-replicating cells was not evident from the processed cryosections. After prolonged autoradiography, another pattern of hybridization was observed, suggesting low levels of viral RNA diffusely distributed throughout most, but not all, follicles. This may represent extracellular virus and/or virus-antibody complexes, but was not consistent with intracellular viral RNA or phagocytosed virus particles. Comparison of hybridization levels in relation to histology suggested a decrease in both the number of HTLV-III replicating cells as well as in the diffusely distributed viral RNA with progression of follicular involution. Our observations indicate that in HTLV-III-infected subjects, lymph node follicles are an important site of virus replication and trapping, suggesting a decisive role of the virus in the characteristic reactive and cytopathogenic changes of HTLV-III-associated lymphadenopathy.