Glymphatic pathways in the gyrencephalic brain.
Glymphatic pathways in the gyrencephalic brain.
复制标题
环脑中的类淋巴通路。
DOI：
10.1177/0271678x21996175
复制
发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Lundgaard I
中科院分区：
文献类型：
--
作者：
Bèchet NB;Shanbhag NC;Lundgaard I
血管周围室是脑脊液(CSF)和间质液之间的交换点，介导脑内溶质的清除，称为淋巴系统，已成为淀粉样蛋白-β等神经毒肽的重要清除途径。然而，淋巴系统的基础科学是建立在啮齿动物研究的基础上的。在这里，我们调查了大脑高度回转的大型哺乳动物是否存在淋巴系统。脑脊液通过血管周围通路进入大脑，这是淋巴功能的标志，可以在整个回脑皮层和皮质下结构中看到，证实了大型哺乳动物的淋巴系统的保护。脑脊液示踪剂的宏观分布跟随脑沟和裂隙，表明这些褶皱增强了脑脊液的弥散。光片显微镜的三维再现显示，猪脑中PVS的流入密度是小鼠的4倍。这证明了在回脑中存在一种先进的溶质运输系统，该系统可以在治疗上用于增强废物的清除。
Identification of the perivascular compartment as the point of exchange between cerebrospinal fluid (CSF) and interstitial fluid mediating solute clearance in the brain, named the glymphatic system, has emerged as an important clearance pathway for neurotoxic peptides such as amyloid-beta. However, the foundational science of the glymphatic system is based on rodent studies. Here we investigated whether the glymphatic system exists in a large mammal with a highly gyrified brain. CSF penetration into the brain via perivascular pathways, a hallmark of glymphatic function, was seen throughout the gyrencephalic cortex and subcortical structures, validating the conservation of the glymphatic system in a large mammal. Macroscopic CSF tracer distribution followed the sulci and fissures showing that these folds enhance CSF dispersion. Three-dimensional renditions from light sheet microscopy showed a PVS influx density 4-fold larger in the pig brain than in mice. This demonstrates the existence of an advanced solute transport system in the gyrencephalic brain that could be utilised therapeutically for enhancing waste clearance.