Lens epithelial, cell apoptosis is an early event in the development of UVB-induced cataract
Lens epithelial, cell apoptosis is an early event in the development of UVB-induced cataract
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DOI：
10.1016/0891-5849(96)02050-3
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发表时间：
1996-01-01
影响因子：
7.4
通讯作者：
Spector, A
中科院分区：
文献类型：
--
作者：
Li, WC;Spector, A
流行病学和实验研究表明，暴露于紫外线可诱发白内障发生。为了研究这种诱导的机制，检查了经 UVB 处理的大鼠晶状体的晶状体上皮细胞的活力。用 8 J/s/m(2) UVB 照射培养的大鼠晶状体 60 分钟，通过末端脱氧核糖核苷酸转移酶 (TdT) 标记和 DNA 片段化分析确定，会触发晶状体上皮细胞凋亡。凋亡的晶状体上皮细胞最初出现在赤道区，然后很快出现在赤道区和中央区。在辐照后孵育期间，凋亡细胞的百分比持续增加。 UVB 孵育 5 小时后，超过 50% 的晶状体上皮细胞凋亡。到24小时，受照射镜片中的所有晶状体上皮细胞均因凋亡而死亡。与该细胞凋亡过程相关的是原癌基因 c-fos 的大量上调。浑浊似乎是随着晶状体上皮细胞死亡而发生的，首先发生在赤道区域，然后发生在中央区域。这对于经典的白内障参数（例如非蛋白硫醇和湿重）也是如此，这些参数仅在明显的上皮细胞凋亡后才会显着改变。总之，这些结果表明 UVB 诱导的晶状体上皮细胞的快速凋亡引发了白内障的发生。
Epidemiological and experimental studies have revealed that exposure to UV can induce cataractogenesis. To investigate the mechanism of this induction, viability of the lens epithelial cells from UVB-treated rat lenses were examined. Irradiation of the cultured rat lenses with 8 J/s/m(2) UVB for 60 min triggers lens epithelial cell apoptosis as determined by terminal deoxyribonucleotide transferase (TdT) labeling and DNA fragmentation assays. The apoptotic lens epithelial cells were initially found in the equatorial region and then quickly appeared in both equatorial and central regions. The percentage of apoptotic cells continuously increased during the postirradiation incubation. After a 5-h post-UVB incubation, more than 50% of the lens epithelial cells were apoptotic. By 24 h, all of the lens epithelial cells in the irradiated lenses were dead through apoptosis. Associated with this apoptotic process is a large upregulation of the proto-oncogene, c-fos. Opacification appears to follow the death of lens epithelial cells occurring first in the equatorial region and then in the central area. This is also true of classical cataract parameters such as non-protein thiol and wet weight, which are significantly modified only after appreciable epithelial cell apoptosis. Together, these results suggest that the rapid apoptotic death of the lens epithelial cells induced by UVB initiates cataract development.