Molecular characterization of Japanese indigenous grape cultivar 'Koshu' (Vitis vinifera) leaf and berry skin during grape development
Molecular characterization of Japanese indigenous grape cultivar 'Koshu' (Vitis vinifera) leaf and berry skin during grape development
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DOI：
10.1007/s11816-009-0095-6
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发表时间：
2009-07-01
影响因子：
2.4
通讯作者：
Takayanagi, Tsutomu
中科院分区：
文献类型：
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作者：
Kobayashi, Hironori;Fujita, Keiko;Takayanagi, Tsutomu
我们研究了日本本土葡萄品种‘Koshu’(Vitis Vinifera)成熟过程中叶片和浆果果皮的转录图谱。在叶片中，基因在花后14周(V1)末大量转录，而61个基因在成熟末(花后20周)表达上调。在浆果果皮中，有67个基因在v1基因末端大量转录，而86个基因在成熟末期表达上调。与生物学过程相关的基因表达在成熟结束时在两个组织中都被激活。在成熟过程中，叶片和浆果果皮中光合作用、糖合成、花青素合成、肉桂酸合成和氨基酸代谢相关基因的表达，以及糖、花青素、肉桂酸和氨基酸的积累。抑制生长素诱导蛋白活性的AUX/IAA家族蛋白的转录产物在浆果果皮中的v-Rison末端表达。在果实成熟末期，降解AUX/IAA家族蛋白的泛素-蛋白酶体系统相关基因的转录产物在果实果皮中大量表达，这表明果皮细胞的扩张受到AUX/IAA家族蛋白的抑制，泛素-蛋白酶体系统通过降解AUX/IAA家族蛋白诱导成熟过程中果皮细胞的扩张。这些转录谱为‘Koshu’葡萄成熟过程中的特性提供了新的信息，可能解释了‘Koshu’葡萄与用于酿酒的欧洲葡萄相比的独特特性，并可能有助于‘Koshu’葡萄品质的改善。
We investigated the transcriptional profiles of Japanese indigenous grape cultivar 'Koshu' (Vitis vinifera) leaf and berry skin during ripening. In leaf, 64 genes were abundantly transcribed at the end of v,raison (14 weeks post-flowering), whereas the expression of 61 genes was upregulated at the end of ripening (20 weeks post-flowering). In berry skin, 67 genes were abundantly transcribed at the end of v,raison, whereas the expression of 86 genes was upregulated at the end of ripening. Gene expression associated with biological processes was activated in both tissues at the end of ripening. The expression of genes associated with photosynthesis, sugar synthesis, anthocyanin synthesis, cinnamic acid synthesis, and amino acid metabolism was observed in leaf and berry skin during ripening, together with the accumulation of sugars, anthocyanins, cinnamic acids, and amino acids. Transcripts of AUX/IAA family proteins that repress the activities of auxin-induced proteins were expressed in berry skin at the end of v,raison. Transcripts of genes related to the ubiquitin-proteasome system that degrades AUX/IAA family proteins were abundantly expressed in berry skin at the end of ripening, suggesting that the expansion of skin cells at v,raison is suppressed by AUX/IAA family proteins, and that the ubiquitin-proteasome system induces the expansion of skin cells during ripening by degrading AUX/IAA family proteins. These transcriptional profiles, which provide new information on the characteristics of 'Koshu' grapevine during ripening, may explain the unique characteristics of 'Koshu' grape in comparison with those of European grapes used for winemaking, and may contribute to the improvement of 'Koshu' grape quality.