In silico and experimental methods revealed highly diverse bacteria with quorum sensing and aromatics biodegradation systems - A potential broad application on bioremediation
In silico and experimental methods revealed highly diverse bacteria with quorum sensing and aromatics biodegradation systems - A potential broad application on bioremediation
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计算机和实验方法揭示了具有群体感应和芳香族生物降解系统的高度多样化的细菌——生物修复的潜在广泛应用
DOI：
10.1016/j.biortech.2013.08.155
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发表时间：
2013-11-01
影响因子：
11.4
通讯作者：
Lin, Xiuchun
中科院分区：
文献类型：
--
作者：
Huang, Yili;Zeng, Yanhua;Lin, Xiuchun
芳香族化合物降解菌与基于酰基高丝氨酸内酯（阿勒）或自诱导物（AI）的群体感应（QS）细菌之间的系统发育重叠在文献中是明显的;然而，具有这两种活性的细菌的多样性从未被精细描述。在NCBI基因组数据库中进行计算机检索发现，超过11%的被调查群体同时携带芳香环羟化双加氧酶（RHO）基因和阿勒/AI合成酶基因。这些细菌分布于10目15科42属78种。这两种基因的水平转移在它们之间很常见。采用富集培养的方法，从海洋、湿地和土壤样品中分离到6株鞘氨醇单胞菌和4株根瘤菌，它们都具有降解菲、芘的能力，并能产生AHL。薄层色谱和气相色谱-质谱分析表明，这些鞘氨醇单胞菌产生多种阿勒分子。这是第一次报告的高度多样化的细菌，窝藏芳香族降解和QS系统。QS法规可能对芳烃生物降解产生广泛的影响，并将成为开发生物修复技术的一个新角度。(C)2013爱思唯尔有限公司保留所有权利。
Phylogenetic overlaps between aromatics-degrading bacteria and acyl-homoserine-lactone (AHL) or autoinducer (AI) based quorum-sensing (QS) bacteria were evident in literatures; however, the diversity of bacteria with both activities had never been finely described. In-silico searching in NCBI genome database revealed that more than 11% of investigated population harbored both aromatic ring-hydroxylating-dioxygenase (RHO) gene and AHL/AI-synthetase gene. These bacteria were distributed in 10 orders, 15 families, 42 genus and 78 species. Horizontal transfers of both genes were common among them. Using enrichment and culture dependent method, 6 Sphingomonadales and 4 Rhizobiales with phenanthrene- or pyrene-degrading ability and AHL-production were isolated from marine, wetland and soil samples. Thin-layer-chromatography and gas-chromatography-mass-spectrum revealed that these Sphingomonads produced various AHL molecules. This is the first report of highly diverse bacteria that harbored both aromatics-degrading and QS systems. QS regulation may have broad impacts on aromatics biodegradation, and would be a new angle for developing bioremediation technology. (C) 2013 Elsevier Ltd. All rights reserved.