INVITRO TUMORICIDAL ACTIVITY OF MACROPHAGES AGAINST VIRUS-TRANSFORMED LINES WITH TEMPERATURE-DEPENDENT TRANSFORMED PHENOTYPIC CHARACTERISTICS
INVITRO TUMORICIDAL ACTIVITY OF MACROPHAGES AGAINST VIRUS-TRANSFORMED LINES WITH TEMPERATURE-DEPENDENT TRANSFORMED PHENOTYPIC CHARACTERISTICS
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DOI：
10.1016/0008-8749(78)90039-4
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发表时间：
1978-01-01
影响因子：
4.3
通讯作者：
POSTE, G
中科院分区：
文献类型：
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作者：
FIDLER, IJ;ROBLIN, RO;POSTE, G
用病毒转化的细胞系研究了靶点对杀肿瘤大鼠和小鼠巨噬细胞破坏的敏感性，其中转化表型的各种元素仅在特定温度下表达。由多瘤病毒的ts 3 [温度敏感性]突变体转化的BHK [幼仓鼠肾]细胞、由SV 40的tsA顺反子突变体转化的大鼠胚胎3 Y1细胞和SV 40转化的小鼠[成纤维细胞] 3 T3细胞的ts-H6-15温度敏感性系在体外被巨噬细胞在允许温度（33 ℃）下杀死。C）或非允许性（39 ° C）。C）转化表型表达的温度。用野生型SV 40或多瘤病毒转化的3 T3、3 Y1和BHK细胞也被33 ℃的杀肿瘤巨噬细胞破坏。C和39度。C，而未转化的3 T3、3 Y1和BHK细胞则无此反应。因此，无论转化细胞是否表达细胞表面LETS蛋白或Forssman抗原，是否表现出允许低剂量植物凝集素凝集的表面变化，是否表达SV 40 T抗原，是否具有低饱和密度或是否表现出密度依赖性的DNA合成抑制，它们都被巨噬细胞杀死。
The susceptibility of targets to destruction by tumoricidal rat and mouse macrophages was studied with virus-transformed cell lines in which various elements of the transformed phenotype are only expressed at specific temperatures. BHK [baby hamster kidney] cells transformed by the ts3 [temperature sensitive] mutant of polyoma virus, rat embryo 3Y1 cells transformed by a ts A cistron mutant of SV40 and the ts-H6-15 temperature-sensitive line of SV40-transformed mouse [fibroblast] 3T3 cells were killed in vitro by macrophages at the permissive (33.degree. C) or nonpermissive (39.degree. C) temperatures for expression of the transformed phenotype. 3T3, 3Y1 and BHK cells transformed by wild-type SV40 or polyoma virus were also destroyed by tumoricidal macrophages at 33.degree. C and 39.degree. C, but untransformed 3T3, 3Y1 and BHK cells were not. Thus, transformed cells are killed by macrophages regardless of whether or not they express cell surface LETS [large external transformation sensitive] protein or Forssman antigen, display surface changes which permit agglutination by low doses of plant lectins, express SV40 T [tumor] antigen, have a low saturation density or exhibit density-dependent inhibition of DNA synthesis.