A grape VvHOS1-interacting HIPP protein (VvHIPP21) negatively regulates cold and drought stress
A grape VvHOS1-interacting HIPP protein (VvHIPP21) negatively regulates cold and drought stress
复制标题
葡萄 VvHOS1 相互作用的 HIPP 蛋白 (VvHIPP21) 对寒冷和干旱胁迫具有负调节作用
DOI：
10.1016/j.envexpbot.2022.105203
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发表时间：
2023-01-02
影响因子：
5.7
通讯作者：
Xu，Weirong
中科院分区：
文献类型：
--
作者：
Zheng，Qiaoling;Yu，Qinhan;Xu，Weirong
重金属相关异戊二烯化植物蛋白（HIPPs）是一类重要的金属伴侣蛋白，在重金属的体内平衡和解毒过程中发挥重要作用。然而，它们在非生物胁迫中的特定调节作用在很大程度上是有限的。在这里，我们证明了来自葡萄属葡萄的aHIPP 21（命名为VvHIPP 21）是RING型E3泛素连接酶HOS 1的一个新的功能组分，该泛素连接酶HOS 1被认为是冷信号传导的关键负调控因子。VvHIPP 21基因在低温和阿坝胁迫下受到抑制，而在干旱胁迫下表达增强，其表达产物定位于细胞质和细胞核中。35 S：VvHIPP 21在拟南芥中的异位表达可以缓解金属离子胁迫以及Cu ~（2+）和Cd ~（2+）的相对毒性。值得注意的是，单独过表达VvHIPP 21或共表达VvHOS 1和VvHIPP 21大大增加了酵母的冷敏感性，如酵母细胞在冷冻应激条件下的存活率所证明的。VvHIPP 21在拟南芥中的过表达通过下调CBF依赖途径中与冷相关的基因而导致冷敏感性。此外，VvHIPP 21过表达的植物表现出减弱的耐旱性，表现为失水率增加，气孔开度扩大，活性氧（ROS）的积累，ABA依赖的干旱响应基因的表达改变。我们的研究结果表明，VvHIPP 21是有利的参与重金属稳态和解毒，但不利地影响冷信号通路和ABA介导的耐旱性。我们的研究结果揭示了植物如何控制生长和发育，以应对重金属和非生物胁迫。
Heavy metal-associated isoprenylated plant proteins (HIPPs) are metallochaperones that contribute significantly to heavy metal homeostasis and detoxification processes. However, their specific regulatory roles in abiotic stresses are largely limited. Here, we show evidence that aHIPP21from Vitis vinifera (named asVvHIPP21) is a novel functional component of the RING-type E3 ubiquitin ligaseHOS1, which is recognized to be a key negative regulator of the cold signaling. VvHIPP21gene is substantially suppressed in response to cold and ABA, but elevated by drought, and its product localized in both the cytoplasm and nucleus. Ectopic expression of35 S:VvHIPP21in Arabidopsis might alleviate the metal ion stress and the relative toxicity of Cu2+and Cd2+, respectively. Notably, overexpression ofVvHIPP21alone or co-expression ofVvHOS1andVvHIPP21greatly increase cold sensitivity in yeast, as evidenced by the survival rate of yeast cells under freezing stress conditions. Overexpression ofVvHIPP21inArabidopsisresulted in cold sensitivity by down-regulating the cold-related genes ofCBF-dependent pathway, relative to wild-type plants. In addition,VvHIPP21-overexpressing plants exhibited attenuated drought tolerance, as shown by an increased water loss rate, expanded stomatal aperture, accumulation of reactive oxygen species (ROS), and altered expression of ABA-dependent drought-responsive genes. Our results show thatVvHIPP21is favorably implicated in heavy metal homeostasis and detoxification, but adversely affects the cold signal pathway and ABA-mediated drought tolerance. Our findings shed light on how plants control growth and development in response to heavy metal and abiotic stresses.