Synergistic photoactivated antimicrobial effects of carbon dots combined with dye photosensitizers
Synergistic photoactivated antimicrobial effects of carbon dots combined with dye photosensitizers
复制标题
DOI：
10.2147/ijn.s183086
复制
发表时间：
2018-01-01
影响因子：
8
通讯作者：
Yang, Liju
中科院分区：
文献类型：
--
作者：
Dong, Xiuli;Bond, Ambrose E.;Yang, Liju
背景：碳量子点（CDots）最近被报道为一类新的可见光激活的抗菌纳米材料。本研究报道了CDots与光敏剂对细菌细胞的协同光活化抗菌相互作用。方法：在可见光照射和不照射1小时的情况下，评价了具有表面钝化分子2，2 '-（亚乙二氧基）双（乙胺）的CDots与不同浓度的光敏剂亚甲基蓝（MB）或甲苯胺蓝（TB）组合对大肠杆菌细胞的抗菌作用。采用微量肉汤稀释棋盘法和等效线图分析确定CDots与MB或TB之间是否存在协同效应。结果表明，单独的CDots在5 μ g/mL的浓度下不显示抗微生物效果，单独的1 μ g/mL MB仅降低1.86 log的活细胞数，但是用5 μ g/mLCDot与1 μ g/mLMB组合的组合处理完全抑制细菌生长，导致6.2log活细胞数减少，表明两者之间的协同相互作用。CDots/TB组合对大肠杆菌的抑菌作用具有相似的协同作用。大肠杆菌细胞。CDots与MB或TB之间的这些协同效应使用棋盘微量稀释法进一步证实，其中分数抑制浓度指数值（0.5）和等效线图分析。这种协同作用还与E.结论：CDots与其他光敏剂联合应用具有协同光活化抗菌作用。这种协同效应可能为开发高效抗菌方法开辟新的策略。
Background: Carbon quantum dots (CDots) have recently been reported as a new class of visible light activated antimicrobial nanomaterials. This study reports the synergistic photoactivated antimicrobial interactions of CDots with photosensitizers on bacterial cells.Methods: The antimicrobial effects of the CDots with surface passivation molecules 2,2'-(ethylenedioxy) bis(ethylamine) in combination with photosensitizer methylene blue (MB) or toluidine blue (TB) at various concentrations were evaluated against Escherichia coli cells with and without 1-hour visible light illumination. The broth microdilution checkerboard method and isobologram analysis were used for determining if synergistic effect existed between CDots and MB or TB.Results: The results showed that CDots alone at a concentration of 5 mu g/mL did not display antimicrobial effects, 1 mu g/mL MB alone only decreased 1.86 log of viable cell numbers, but the combination treatment with 5 mu g/mL CDots combined with 1 mu g/mL MB completely inhibited bacteria growth, resulted in 6.2 log viable cell number reduction, suggesting synergistic interaction between the two. The antimicrobial effects of CDots/TB combination exhibited similarly synergistic effects on E. coli cells. These synergistic effects between CDots and MB or TB were further confirmed using the checkerboard microdilution methods, where the fractional inhibitory concentration index value (0.5) and the isobologram analyses. The synergistic interactions were also correlated to the increased generation of intracellular reactive oxygen species in E. coli cells upon the combination treatments of CDots/MB or CDots/TB.Conclusion: The study demonstrated the synergistic photoactivated antimicrobial effects of CDots in combination with other photosensitizers. Such synergistic effect may open new strategies for developing highly effective antimicrobial methods.