Thyroid hormone receptor gene expression in first trimester human fetal brain.
Thyroid hormone receptor gene expression in first trimester human fetal brain.
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甲状腺激素受体基因在妊娠早期人胎儿大脑中的表达。
DOI：
10.1210/jcem.85.7.6766
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Roger Ekins
中科院分区：
文献类型：
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作者：
Joseph Iskaros;Mark Pickard;Ian M. Evans;Arun Sinha;Paul Hardiman;Roger Ekins
母体甲状腺激素在妊娠早期转移给胎儿，并被认为调节大脑发育。胎儿脑中存在甲状腺激素核受体（TR）蛋白，但其同型组成尚不清楚。因此，我们通过半定量逆转录-聚合酶链式反应分析研究了妊娠早期人类胎儿大脑（n = 9）中TR亚型和相关剪接变异的个体发生。从妊娠8.1周（wg）开始检测TRbeta1、TRalpha1和c-erbAalpha2亚型的表达。c-erbAalpha2引物组检测到另一个截断物种，与先前在大鼠中描述的c-erbAalpha3剪接变体一致。所有c- erbaα衍生转录本在8.1至13.9 wg之间协调表达并增加（约8倍）。观察到的TRbeta1的发病模式更为复杂，表明其最低点在8.4 - 12.0 wg之间。这些发现表明TRalpha1亚型在妊娠早期人类胎儿大脑发育过程中介导母体甲状腺激素的作用。
Maternal thyroid hormone is transferred to the fetus early in pregnancy and is postulated to regulate brain development. Thyroid hormone nuclear receptor (TR) proteins are present in fetal brain, but their isoformal composition is unknown. We therefore investigated the ontogeny of TR isoforms and related splice variants in first trimester human fetal brain (n = 9) by semi-quantitative reverse transcriptase-polymerase chain reaction analysis. Expression of the TRbeta1, TRalpha1 and c-erbAalpha2 isoforms was detected from 8.1 weeks gestation (wg). An additional truncated species was detected with the c-erbAalpha2 primer set, consistent with the c-erbAalpha3 splice variant previously described in the rat. All c-erbAalpha-derived transcripts were co-ordinately expressed and increased (ca. 8-fold) between 8.1 and 13.9 wg. A more complex ontogenic pattern was observed for TRbeta1, suggestive of a nadir between 8.4 and 12.0 wg. These findings point to an important role for the TRalpha1 isoform in mediating maternal thyroid hormone action during first trimester human fetal brain development.