Bone morphogenetic protein-3b (BMP-3b) gene expression is correlated with differentiation in rat calvarial osteoblasts
Bone morphogenetic protein-3b (BMP-3b) gene expression is correlated with differentiation in rat calvarial osteoblasts
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DOI：
10.1006/bbrc.1999.0341
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发表时间：
1999-03-16
影响因子：
3.1
通讯作者：
Kangawa, K
中科院分区：
文献类型：
--
作者：
Hino, J;Matsuo, H;Kangawa, K
BMP-3b（也称为GDF-10）是最近在大鼠股骨组织中发现的一种新的bmp -3相关蛋白。研究了BMP-3b基因在成骨细胞中的表达及其在长时间培养、BMP-2和转化生长因子β 1 （tgf - β 1）中的调控作用。BMP-3b基因在大鼠颅骨成骨细胞中高表达，而在成骨细胞系（MC3T3-E1和U2-OS）中不表达，BMP-3b mRNA在长时间培养成骨细胞分化过程中升高，并与碱性磷酸酶（ALPase）活性升高有关。当原代成骨细胞中加入ALPase活性增强剂BMP-2时，BMP-3b mRNA在24 h后增加了6.9倍。相比之下，抑制ALPase活性的tgf - β 1能迅速完全抑制BMP-3b的基因表达。BMP-3 mRNA的调控与BMP-3b不同，尽管两者具有81%的同源性。这些发现表明BMP-3b基因表达受成骨细胞分化调控，BMP-3b在高分化成骨细胞中发挥功能。(C) 1999学术出版社。
BMP-3b (also called GDF-10) is a novel BMP-3-related protein recently discovered in rat femur tissue. Gene expression of BMP-3b in osteoblastic cells and its regulation by prolonged culture, BMP-2 and transforming growth factor beta 1 (TGF-beta 1) were examined, The BMP-3b gene was highly expressed in rat osteoblasts obtained from calvarial bones but not in the osteoblastic cell lines (MC3T3-E1 and U2-OS), BMP-3b mRNA increased during osteoblastic differentiation in prolonged culture and was associated with increased alkaline phosphatase (ALPase) activity. When BMP-2, an enhancer of ALPase activity, was added to the primary osteoblast culture, BMP-3b mRNA increased 6.9-fold after 24 h. In contrast, TGF-beta 1 treatment, which suppresses ALPase activity, rapidly and completely inhibited gene expression of BMP-3b. The regulation of BMP-3 mRNA differed from that of BMP-3b, even though both proteins share 81% identity. These findings indicate that BMP-3b gene expression is regulated by osteoblastic differentiation and BMP-3b functions in highly differentiated osteoblasts. (C) 1999 Academic Press.