Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots
Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots
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DOI：
10.1039/c5an00933b
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发表时间：
2015-01-01
期刊：
影响因子：
4.2
通讯作者：
Cooper, Helen J.
中科院分区：
文献类型：
--
作者：
Griffiths, Rian L.;Dexter, Alex;Cooper, Helen J.
液体萃取表面分析（LESA）是一种表面采样技术，允许直接从生物基质中对各种分析物进行电喷雾质谱分析。在这里，我们提出了LESA质谱法与高场非对称波形离子迁移谱法（FAIMS）相结合，用于分析滤纸上的干血斑。在工作流程中引入FAIMS可实现脂质和蛋白质分子类别的气相分离，从而可从单个提取样品中分析血红蛋白和一系列脂质（磷脂酰胆碱或磷脂酰乙醇胺和鞘磷脂物质）。这项工作对多种分析物的多重临床测定具有影响。
Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.