Applying a Progesterone Assay to Faecal Samples Collected from Sows during the Oestrous Cycle
Applying a Progesterone Assay to Faecal Samples Collected from Sows during the Oestrous Cycle
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对发情周期期间从母猪采集的粪便样本进行黄体酮测定
DOI：
10.1111/j.1439-0531.1995.tb00613.x
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发表时间：
1995
影响因子：
1.7
通讯作者：
A. Dalin
中科院分区：
文献类型：
--
作者：
F. Hultén;B. Zhang;M. Forsberg;A. Dalin
从断奶后第二天至断奶后第3次发情期间，隔日采集6头经产母猪的粪便和血浆样品。在允许血浆浓度的变化滞后2天以反映在粪便浓度中之后，粪便“孕酮”浓度（提取的孕酮和孕酮代谢物的总量）与血浆孕酮浓度的相关性平均为0.86（范围0.73-0.93）。结果还表明，通过粪便样品的分析可以准确地区分黄体期和卵泡期。粪便中[3 H]孕酮的回收率较低，对1份提取样品的系列稀释液分析未显示平行性，表明该方法的有效性仅基于与血浆孕酮浓度的高度相关性。
Faecal and plasma samples were collected from 6 multiparous sows on alternate days from the day after weaning until the 3rd oestrus after weaning. After allowing a 2-day lag for changes in plasma concentration to be reflected in faecal concentration, the correlation of faecal 'progestin' concentration (total of progesterone and progesterone metabolites extracted) with plasma progesterone concentration averaged 0.86 (range 0.73-0.93). The results also showed that luteal and follicular phases could be accurately distinguished by the assay of faecal samples. Recovery of [3H]progesterone from faeces was low, and analysis of serial dilutions on 1 extracted sample did not show parallelism, demonstrating that the validity of the method is based only on the high correlation with plasma progesterone concentrations.
快速提取粪便类固醇，用于测量自由放养的黄色狒狒（Papio cynocephalus cynocephalus）的生殖周期和早期妊娠。
DOI：
10.1530/jrf.0.0920415
发表时间：
1991
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Wasser,SK;Monfort,SL;Wildt,DE
通讯作者：
Wildt,DE