Carboxybetaine polymer-protected gold nanoparticles: High dispersion stability and resistance against non-specific adsorption of proteins
Carboxybetaine polymer-protected gold nanoparticles: High dispersion stability and resistance against non-specific adsorption of proteins
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DOI：
10.1002/macp.200600652
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发表时间：
2007-04
影响因子：
2.5
通讯作者：
Kazuhiro Matsuura;K. Ohno;S. Kagaya;H. Kitano
中科院分区：
文献类型：
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作者：
Kazuhiro Matsuura;K. Ohno;S. Kagaya;H. Kitano
1-羧基-N，N-dimethyl-N-(2‘-methacryloyloxyethyl)methanaminium内盐(CMB)由2-溴异丁基引发的二硫键引发聚合而成。以二硫代羧基甜菜碱聚合物(DT-PCMB)为原料，在不同摩尔比的DT-PCMB和HAuCl4存在下，还原四氯金酸(HAuCl4)，制备了PCMB保护的金纳米粒子(PCMB-AuNPs)。随着DT-PCMB的浓度和相对分子质量的降低，PCMB-AuNPs中的金核尺寸有增大的趋势。PCMB-AuNPs具有很高的分散稳定性，并对蛋白质(牛血清白蛋白、人血清白蛋白、溶菌酶和细胞色素c)的非特异性吸附具有抵抗力。因此，DT-PCMB是一种非常适合制备惰性AuNPs的稳定配体，PCMB-AuNPs将在生物医学应用中得到应用。
1-Carboxy-N,N-dimethyl-N-(2'-methacryloyloxyethyl)methanaminium inner salt (CMB) was polymerized by ATRP initiated with a disulfide difunctionalized by 2-bromoisobutyryl groups. The disulfide-carrying carboxybetaine polymer (DT-PCMB) was used for the preparation of PCMB-protected gold nanoparticles (PCMB-AuNPs) obtained by the reduction of hydrogen tetrachloroaurate (HAuCl 4 ) in the presence of the DT-PCMB of different molecular weights at different molar ratios of DT-PCMB and HAuCl 4 . The sizes of gold cores in the PCMB-AuNPs tended to increase upon decreasing concentration and molecular weight of the DT-PCMB. The PCMB-AuNPs possessed a high dispersion stability, and showed a resistance against non-specific adsorption of proteins (bovine serum albumin, human serum albumin, lysozyme, and cytochrome c). Therefore, DT-PCMB is a quite suitable stabilizing ligand to prepare inert AuNPs and the PCMB-AuNPs will be useful in biomedical applications.