Large scale blood group genotyping
Large scale blood group genotyping
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DOI：
10.1016/j.tracli.2007.04.011
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发表时间：
2007-05-01
影响因子：
1.7
通讯作者：
Avent, Neil D.
中科院分区：
文献类型：
--
作者：
Avent, Neil D.
自20世纪90年代以来，已通过测定基因型来确定预测的血型表型。这是由于群抗原表达而发展的，群抗原表达始于1990年，随着ABO和RH系统围绕血液g的分子基础的信息的快速积累，现在已经导致29个血型中的28个的分子描述。血型基因分型目前主要用于胎儿血型不相容性和多次输血患者的评估。这两种临床情况要么是危险的，要么是技术上困难的，分别从血清学上定义。随着大规模基因分型平台的同时发展，现在已经允许将其应用于血型基因型测定。在本文中，我描述了一些最近发表的工作，已经证明，大规模的基因分型方法是可行的。这些方法可能会导致更有效的血液库存和患者交叉配血的管理，减少在关键的输血前阶段对血清学的依赖。最有可能的是，大规模的研究，可能涉及许多欧盟和北美的血液供应商，可能会推动这项技术的引入，并说服红细胞血清学家，这种方法可以使他们的工作更加集中。(C)2007年，Elsevier Masson SAS。All rights reserved.
Determination of predicted blood group phenotype by determination of genotype has been performed since the 1990s. This evolved due to the,roup antigen expression, which started in 1990 with ABO and RH systems rapid accrual of information surrounding the molecular basis of blood g and has now resulted in the molecular description of 28 of the 29 blood groups. Blood group genotyping is currently performed mostly for fetal blood group incompatibility and for assessment of multi-transfused patients. Both of these clinical scenarios are either dangerous or technically difficult, respectively to define serologically. With the simultaneous development of mass scale genotyping platforms it has now permitted the application of them to blood group genotype determination. In this paper, I describe some recently published work that has demonstrated that mass scale genotyping approaches are feasible. These approaches may lead to more effective management of blood stocks and patient cross-matching by reducing the dependence on serology during the time critical pre-transfusion phase. It is most probable that large scale studies, perhaps involving many European Union and North American based blood suppliers, may drive the introduction of this technology and convince red cell serologists that this approach may allow their work to be more focussed. (C) 2007 Elsevier Masson SAS. All rights reserved.