3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model.
3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model.
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DOI：
10.1016/j.biomaterials.2015.07.039
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发表时间：
2015-10
期刊：
影响因子：
14
通讯作者：
Grande-Allen KJ
中科院分区：
文献类型：
--
作者：
Puperi DS;Balaoing LR;O'Connell RW;West JL;Grande-Allen KJ
需要生理相关的体外模型来研究疾病的进展，开发和筛选潜在的疾病治疗干预措施。尤其是心脏瓣膜病，由于缺乏对导致疾病的细胞机制的了解，因此没有早期干预或非侵入性治疗。在这里，我们建立了一个新颖的、可定制的合成水凝胶平台，可以用于研究细胞与细胞之间的相互作用以及导致瓣膜疾病的因素。在3D共培养中，结合在支架中的空间定位的细胞黏附配体促进瓣膜间质细胞和瓣膜内皮细胞的生长和组织。这两种类型的细胞都保持了表型、内稳态功能，并产生了带状定位的细胞外基质。该模型扩展了体外研究的能力，提供了一个与生理上相关的空间排列的细胞进行直接接触共培养的平台。
Physiologically relevant in vitro models are needed to study disease progression and to develop and screen potential therapeutic interventions for disease. Heart valve disease, in particular, has no early intervention or non-invasive treatment because there is a lack of understanding the cellular mechanisms which lead to disease. Here, we establish a novel, customizable synthetic hydrogel platform that can be used to study cell-cell interactions and the factors which contribute to valve disease. Spatially localized cell adhesive ligands bound in the scaffold promote cell growth and organization of valve interstitial cells and valve endothelial cells in 3D co-culture. Both cell types maintained phenotypes, homeostatic functions, and produced zonally localized extracellular matrix. This model extends the capabilities of in vitro research by providing a platform to perform direct contact co-culture with cells in their physiologically relevant spatial arrangement.