Spatial distribution and correlation of adipocytes and mast cells in superficial fascia in rats
Spatial distribution and correlation of adipocytes and mast cells in superficial fascia in rats
复制标题
大鼠浅筋膜脂肪细胞和肥大细胞的空间分布及相关性
DOI：
10.1007/s00418-019-01812-5
复制
发表时间：
2019-12-01
影响因子：
2.3
通讯作者：
Xu, Guoheng
中科院分区：
文献类型：
--
作者：
Zhang, Dandan;Dong, Yingyue;Xu, Guoheng
浅筋膜中含有丰富的前脂肪细胞，具有自发和诱导分化的能力，被认为是脂肪细胞的新来源。本研究旨在定量评价大鼠浅筋膜中脂肪细胞和肥大细胞的空间分布及其相关性。全景图像由甲苯胺蓝染色的全封筋膜获得。生长期大鼠浅筋膜脂肪细胞逐渐增多。筋膜内出现大量肥大细胞，并伴有大量分泌颗粒的脱颗粒和胞吐作用，部分分化的脂肪细胞和成熟的脂肪细胞出现在筋膜内。组织学方差均值比和Morisita离散指数定量分析表明，筋膜内肥大细胞和脂肪细胞均呈独立的簇状分布，而非随机或均匀分布。斯皮尔曼相关系数显示肥大细胞和脂肪细胞的空间聚集分布彼此正相关，并且与筋膜中脂肪细胞的数量和大小以及脂肪形成区域的增加相关。形态计量学分析表明，在大鼠生长发育过程中，筋膜脂肪细胞和肥大细胞之间存在着很强的相关性。与4周相比，8周时相关系数显著增加，与生长期大鼠筋膜脂肪细胞数量和大小的增加趋势一致。这一发现首次对筋膜脂肪细胞和肥大细胞的空间分布和相关性进行了定量组织学分析，为进一步探讨筋膜肥大细胞和脂肪细胞之间的空间和功能相互作用提供了组织细胞学基础。为进一步研究筋膜及筋膜相关结缔组织的生理和病理提供了资料。
Superficial fascia has abundant preadipocytes capable of spontaneous and induced differentiation and is thought to be a novel origin of adipocytes. This study aimed to quantitatively evaluate the spatial distribution and correlation of adipocytes and mast cells in rat superficial fascia. Panoramic images were obtained from whole-mounted fascia stained by toluidine blue. Adipocytes increased gradually in superficial fascia of growing rats. Abundant mast cells, with the degranulation and exocytosis of abundant secretory granules, appeared in fascia where partially differentiating adipocytes and mature adipocytes occurred. Quantitative histological analysis by variance–mean ratio and Morisita index of dispersion indicated that both mast cells and adipocytes in fascia were distributed individually in cluster, but not random or uniform. Spearman’s correlation coefficient revealed that the spatial cluster distributions of mast cells and adipocytes positively correlated with each other and correlated with increased number and size of adipocytes and adipogenic areas in fascia. Morphometry analysis indicated the strong correlation between fascial adipocytes and mast cells during the periods of rat growth. The correlation coefficient increased significantly at 8 weeks compared to 4 weeks, consistent with the increasing trend of the number and size of fascia adipocytes in growing rats. This finding provided the first quantitative histological analysis for the spatial distribution and correlation of fascia adipocytes and mast cells, which could be the histocytological basis for further exploring spatial and functional interactions between fascial mast cells and adipocytes. Also, the present data were informative for the research on physiologies and pathologies of fascia and fascia-related connective tissues.