Nutrigenomic Role of Acetate and β-Hydroxybutyrate in Bovine Mammary Epithelial Cells
Nutrigenomic Role of Acetate and β-Hydroxybutyrate in Bovine Mammary Epithelial Cells
复制标题
乙酸和β-羟基丁酸在牛乳腺上皮细胞中的营养基因组作用
DOI：
10.1089/dna.2019.4783
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发表时间：
2020-01-02
影响因子：
3.1
通讯作者：
Lin, Ye
中科院分区：
文献类型：
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作者：
Song, Shuyuan;Jiang, Minghui;Lin, Ye
乙酸和β-羟基丁酸（BHBA）是奶牛乳腺脂肪酸（FA）从头合成的主要底物。为了研究在乳脂产生过程中乙酸盐和BHBA在牛乳腺上皮细胞中的营养基因组作用，使用RNA测序（RNA-seq）转录组学分析来鉴定乙酸盐和BHBA处理的细胞（高乳脂细胞）和对照细胞之间的差异表达基因（DEG）。在高乳脂细胞和对照细胞之间共鉴定出625个DEG（358个上调和267个下调）。基因本体富集分析显示，高乳脂肪细胞中表达上调的基因主要涉及脂质生物合成过程、类固醇生物合成过程、氧化还原过程、受体结合过程、囊泡和小分子生物合成过程。下调的基因主要与免疫应答、细胞因子产生、生物学过程的负调控和肽基苏氨酸修饰有关。途径分析表明，高乳脂肪细胞中FA代谢和类固醇合成的上调基因显著富集，而凋亡的下调基因富集。这项工作提供了一个配置文件的基因表达变化，发生在乙酸和BHBA诱导的牛奶脂肪合成在牛乳腺上皮细胞，这进一步加深了我们的了解脂质代谢的分子调控。
Acetate and beta-hydroxybutyrate (BHBA) are the predominant substrates for de novo fatty acid (FA) synthesis in mammary gland of dairy cow. To investigate the nutrigenomic role of acetate and BHBA in bovine mammary epithelial cells during milk fat production, RNA sequencing (RNA-seq) transcriptomic analysis was used to identify differentially expressed genes (DEGs) between acetate- and BHBA-treated cells (high-milk fat cells) and control cells. A total of 625 DEGs (358 upregulated and 267 downregulated) were identified between the high-milk fat cells and control cells. Gene ontology enrichment analysis revealed that the upregulated genes in high-milk fat cells were mainly involved in lipid biosynthetic process, steroid biosynthetic process, oxidation-reduction process, receptor binding, and vesicle and small molecule biosynthetic process. The downregulated genes were mainly associated with immune response, cytokine production, negative regulation of biological process, and peptidyl-threonine modification. Pathway analysis indicated that FA metabolism and steroid biosynthesis were significantly enriched for the upregulated genes in the high-milk fat cells, while apoptosis was enriched for the downregulated genes. This work provides a profile of gene expression changes that occur during acetate- and BHBA-induced milk fat synthesis in bovine mammary epithelial cells, which furthers our understanding of the molecular regulation of lipid metabolism.