Expression and localization of VEGFR-2 in hair follicles during induced hair growth in mice
Expression and localization of VEGFR-2 in hair follicles during induced hair growth in mice
复制标题
小鼠毛发诱导生长过程中毛囊中 VEGFR-2 的表达和定位
DOI：
10.1007/s00403-018-1843-7
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发表时间：
2018-09-01
影响因子：
3
通讯作者：
Zheng, Min
中科院分区：
文献类型：
--
作者：
Wu, Xian-Jie;Jing, Jing;Zheng, Min
最近，VEGFR-2不仅在血管和淋巴管内皮细胞中被检测到，而且在一些非血管内皮细胞中也被检测到，特别是在人的毛囊、皮脂腺和汗腺中。此外，VEGFR-2已被证实在毛囊角质形成细胞的调控中发挥直接作用，而不仅仅是血管生成。为探讨VEGFR-2激活在毛囊周期调控中的作用，采用免疫荧光方法检测拔毛诱导的小鼠背部毛发周期中VEGFR-2的表达。我们观察到在生长期II和IV期毛囊中VEGFR-2的表达明显强于生长期VI期、退化期和休止期。在生长期，VEGFR-2在毛囊的角质形成细胞束上有强烈的染色。在生长期，VEGFR-2在ORS、IRS和毛球中呈强阳性表达，在ORS和毛球中有中等程度的表达，而在生长期VI中，在IRS中表达最强。在TELGEN期间，我们在与表皮相邻的生殖细胞、帽子和杆状毛发中检测到VEGFR-2。综上所述，VEGFR-2在小鼠背部毛囊中有表达，并在毛发周期过程中表达变化，提示VEGFR-2可能在小鼠背部毛囊的毛发周期调控中发挥作用。
Recently, VEGFR-2 has been detected not only in vascular and lymphatic endothelial cells but also in some non-vascular endothelial cells, particularly human hair follicles, sebaceous glands, and sweat glands. In addition, VEGFR-2 has been confirmed to play direct roles in hair follicle keratinocyte regulation beyond simply angiogenesis. To elucidate whether VEGFR-2 activation plays a role in hair follicle cycling regulation, immunofluorescence of VEGFR-2 expression was performed during hair cycling of the dorsum of the mouse induced by hair plucking. We observed that staining for VEGFR-2 in hair follicles during anagen II and IV was much stronger than during anagen VI, catagen and telogen. During anagen II, intense staining for VEGFR-2 was observed on the keratinocyte strands of the hair follicle. Subsequently, we detected intense staining for VEGFR-2 in the ORS, IRS and hair bulb during anagen IV. Moderate staining for VEGFR-2 was detected in the ORS and hair bulb, but staining was most intense in IRS during anagen VI. During catagen, staining for VEGFR-2 in the IRS remained intense, while staining in the ORS and hair bulb was significantly weakened and was negative in the dermal papilla. During telogen, we detected VEGFR-2 in germ cells, cap, and club hair adjoining the epidermis. In conclusion, VEGFR-2 was expressed on the hair follicles of the dorsum of the mouse and varied in expression on the mouse hair follicles during hair cycling, suggesting that VEGFR-2 may exert roles in hair cycle regulation in hair follicles on the dorsum of mice.