Global gene expression profile of human cord blood-derived CD133+ cells
Global gene expression profile of human cord blood-derived CD133+ cells
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DOI：
10.1634/stemcells.2005-0185
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发表时间：
2006-03-01
期刊：
影响因子：
5.2
通讯作者：
Partanen, Jukka
中科院分区：
文献类型：
--
作者：
Jaatinen, Taina;Hemmoranta, Heidi;Partanen, Jukka
人脐血来源的CD 133（+）细胞具有原始造血细胞的特性，可作为造血干细胞移植中CD 34（+）细胞的替代细胞。为了在遗传水平上表征CD 133（+）细胞群体，使用寡核苷酸微阵列进行CD 133（+）细胞的全局表达分析。通过免疫磁性选择从四个新鲜CB单元中纯化CD 133（+）细胞。所有四个CD 133（+）样品在其基因表达模式中显示出显著的相似性，而它们与CD 133（-）对照样品明显不同。总共有690个转录本在CD 133（+）和CD 133（-）细胞之间差异表达。其中CD 133（+）细胞393个增加，297个减少。在与代谢、细胞生理过程、细胞通讯和发育相关的基因中发现了最高的过表达。鉴定了一组仅在CD 133（+）细胞群中表达的257个转录物。使用集落形成单位（CFU）测定来检测所研究的细胞群体中存在的前体的克隆后代。结果表明，CD 133（+）细胞表达原始标记，并具有克隆形成祖细胞的能力。本研究提供了人CD 133（+）细胞的基因表达谱。它提出了一组基因，可用于解开CD 133（+）细胞群的特性，假设是高度富集的HSC。
Human cord blood (CB)-derived CD133(+) cells carry characteristics of primitive hematopoietic cells and proffer an alternative for CD34(+) cells in hematopoietic stem cell (HSC) transplantation. To characterize the CD133(+) cell population on a genetic level, a global expression analysis of CD133(+) cells was performed using oligonucleotide microarrays. CD133(+) cells were purified from four fresh CB units by immunomagnetic selection. All four CD133(+) samples showed significant similarity in their gene expression pattern, whereas they differed clearly from the CD133(-) control samples. In all, 690 transcripts were differentially expressed between CD133(+) and CD133(-) cells. Of these, 393 were increased and 297 were decreased in CD133(+) cells. The highest overexpression was noted in genes associated with metabolism, cellular physiological processes, cell communication, and development. A set of 257 transcripts expressed solely in the CD133(+) cell population was identified. Colony-forming unit (CFU) assay was used to detect the clonal progeny of precursors present in the studied cell populations. The results demonstrate that CD133(+) cells express primitive markers and possess clonogenic progenitor capacity. This study provides a gene expression profile for human CD133(+) cells. It presents a set of genes that may be used to unravel the properties of the CD133(+) cell population, assumed to be highly enriched in HSCs.