Mechanical contributions to astrocyte adhesion using a novel in vitro model of catheter obstruction
Mechanical contributions to astrocyte adhesion using a novel in vitro model of catheter obstruction
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DOI：
10.1016/j.expneurol.2009.12.027
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发表时间：
2010-04-01
影响因子：
5.3
通讯作者：
McAllister, James P., II
中科院分区：
文献类型：
--
作者：
Harris, Carolyn A.;Resau, James H.;McAllister, James P., II
通过分流系统引流和转移脑脊液（CSF）是脑积水最常见的治疗方法。但是高达61%的患者会出现由于导管的组织阻塞而引起的并发症。尽管分流系统在抵抗哺乳动物细胞粘附方面已经经历了有限的技术进步，临床研究中的高内在变异性和在维持脑积水中观察到的环境条件的同时不能预测体外宿主细胞的慢性粘附我们设计了脑积水分流导管生物反应器（HSCB），以在CSF压力、脉动速率和流速的实验操作条件下测量炎性细胞粘附。在20小时的时间内，通过脉动流系统灌注星形胶质细胞，并与静态细胞培养条件下的结果进行了比较。在增加流速（0 2 5和0 30 mL/min）的条件下，并且在升高的压力和脉动速率的条件下观察到粘附增加的趋势，因为HSCB比静态细胞培养更准确地代表生理条件。我们的结果表明，标准的静态细胞培养技术不足以模拟脑积水治疗中使用的导管上的炎性细胞粘附，并且脑室微环境的变化可以改变细胞粘附的机制。HSCB代表了相关的测试系统，并且是用于分析分流导管的细胞粘附和闭塞的有效模型系统（C）。2010 Elsevier Inc保留所有权利。
Drainage and diversion of cerebrospinal fluid (CSF) through Shunt systems is the most common treatment for hydrocephalus. but complications due to tissue obstruction of the catheter occur in up to 61% of patients Although Shunt systems have undergone limited technological advancements to resist mammalian cell adhesion, there is a need to further reduce adhesion that can exacerbate obstruction The high intrinsic variability in clinical studies and an inability to predict chronic adhesion of host cells in vitro while maintaining the environmental conditions observed in hydrocephalus have impeded progress We designed the hydrocephalus shunt catheter bioreactor (HSCB) to measure inflammatory cell adhesion tinder experimentally manipulated conditions of CSF pressure, pulsation rate, and flow rates For a 20-h period, astrocytes were perfused through the pulsatile flow system, and adhesion on silicone catheters was recorded These results were compared with those obtained under static cell Culture conditions Astrocyte adhesion was significantly increased under conditions of increased flow rate (0 25 and 0 30 mL/min). and a trend toward increased adhesion was observed under conditions of elevated pressure and pulsation rate Because the HSCB represents physiologic conditions more accurately than static cell culture. our results suggest that standard static cell culturing techniques are insufficient to model inflammatory cell adhesion on catheters used in the treatment of hydrocephalus and that changes to the ventricular microenvironment can alter the mechanisms of cellular adhesion The HSCB represents a relevant test system and is an effective model system for the analysis of cellular adhesion and occlusion Of Shunt catheters (C) 2010 Elsevier Inc All rights reserved.