Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase
Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase
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DOI：
10.1016/s1004-9541(09)60188-9
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发表时间：
2011-02-01
影响因子：
3.8
通讯作者：
Li Chun
中科院分区：
文献类型：
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作者：
Liu Yan;Tao Jing;Li Chun
种子萌发试验和番茄幼苗试验表明，枯草芽孢杆菌SL-13能促进番茄的发芽和幼苗生长。番茄幼苗鲜重和干重分别增加42.86%和18.75%。SL-13在温室和田间对番茄根瘤菌病的防治效果分别为20.65%和35.23%。菌株SL-13的培养上清液对植物病原真菌茄枯丝核菌的生长有明显的抑制作用。体外实验检测其主要抗真菌蛋白为几丁质酶。采用DEAE-Sepharose快速流动离子交换柱层析和Sephadex G-75凝胶过滤纯化几丁质酶，进一步进行表征。几丁质酶活性的最佳pH和温度分别为7.0℃和50℃。结果表明，该酶在pH 5 ~ 9、40 ~ 60℃条件下稳定，在121℃、0.11 MPa条件下培养20 min，酶活性保持70%，且对蛋白酶K和紫外线辐射不敏感。因此适合在相对不稳定的环境中进行有效的生物防治。
The seed germination and tomato seedling tests showed that Bacillus subtilis SL-13 could promote the sprouting and seedling growth of tomato. The fresh and dry weight of tomato seedlings increased 42.86% and 18.75%, respectively. The control efficacies of the SL-13 to tomato Rhizoctonia rot were 20.65% and 35.23% in the greenhouse and field, respectively. The growth of the plant-pathogenic fungus Rhizoctonia solani was considerably inhibited in the presence of the strain SL-13 culture supernatant. The main antifungal protein was detected to be chitinase through vitro assay. The chitinase was purified with DEAE-Sepharose fast flow ion exchange column chromatography and Sephadex G-75 gel filtration for further characterization. The optimal pH and temperature for the chitinase activity were 7.0 and 50 degrees C, respectively. It was demonstrated that the enzyme was stable at pH 5-9 and 40-60 degrees C. 70% of the enzyme activity was retained when incubated at 121 degrees C and 0.11 MPa for 20 min, and the enzyme was not sensitive to protease K and ultraviolet radiation. Thus it is suitable for effective biological control in relatively unstable environment.