Intracellular esterase activity in living cells may distinguish between metastatic and tumor-free lymph nodes
Intracellular esterase activity in living cells may distinguish between metastatic and tumor-free lymph nodes
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DOI：
10.1007/s10585-007-9135-1
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发表时间：
2008-05-01
影响因子：
4
通讯作者：
Deutsch, Mordechai
中科院分区：
文献类型：
--
作者：
Afrimzon, Elena;Deutsch, Assaf;Deutsch, Mordechai
背景乳腺癌检测的主要临床问题之一是标准程序检测不到的隐匿性淋巴结转移的发生率相对较高。由于乳腺癌分期的确定决定了后续的治疗，因此这种“低诊断”会导致治疗不充分，从而对患者的结局和生存不利。本研究的目的是探讨乳腺癌患者转移淋巴结和无瘤淋巴结活细胞的功能代谢特征。方法我们的方法是基于活细胞的能力，水解荧光素二乙酸酯（FDA）的细胞内酯酶和FDA水解率与特定的细胞状态，在生理和病理条件下的关联。结果转移性淋巴结和非转移性淋巴结的淋巴结细胞利用FDA的能力在总体上存在显著差异，个体患者转移性淋巴结和非转移性淋巴结的淋巴结细胞利用FDA的能力也存在显著差异。来自转移性淋巴结的细胞具有更高的FDA水解能力，并且在自体肿瘤组织（tt）的额外激活后增加了这种活性。FDA水解率的增加与活化的T淋巴细胞和抗原呈递细胞（APC）之间的关联。结论本研究结果有助于预测腋窝淋巴结隐匿性转移的风险，减少腋窝淋巴结检查的假阴性结果。
Background One of the major clinical problems in breast cancer detection is the relatively high incidence of occult lymph node metastases undetectable by standard procedures. Since the ascertainment of breast cancer stage determines the following treatment, such a "hypo-diagnosis" leads to inadequate therapy, and hence is detrimental for the outcome and survival of the patients. The purpose of our study was to investigate functional metabolic characteristics of living cells derived from metastatic and tumor-free lymph nodes of breast cancer (BC) patients. Methods Our methodology is based on the ability of living cells to hydrolyze fluorescein diacetate (FDA) by intracellular esterases and on the association of FDA hydrolysis rates with a specific cell status, both in physiological and pathological conditions. Results The present study demonstrates a significant difference in the ability to utilize FDA by lymph node cells derived from metastatic and tumor-free lymph nodes in general average, as well as in the metastatic and tumor-free lymph nodes of individual patients. Cells from metastatic lymph nodes had a higher capacity for FDA hydrolysis, and increased this activity after additional activation by autologous tumor tissue (tt). The association between increased FDA hydrolysis rate and activated T lymphocytes and antigen-presenting cells (APC) was shown. Conclusion The results of the present study may contribute to predicting the risk of involvement of seemingly "tumor-free" axillary lymph nodes in occult metastatic processes, and to reducing false-negative results of axillary examination.