BINDING OF CRYPTOCOCCUS-NEOFORMANS BY HUMAN CULTURED MACROPHAGES - REQUIREMENTS FOR MULTIPLE COMPLEMENT RECEPTORS AND ACTIN
BINDING OF CRYPTOCOCCUS-NEOFORMANS BY HUMAN CULTURED MACROPHAGES - REQUIREMENTS FOR MULTIPLE COMPLEMENT RECEPTORS AND ACTIN
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DOI：
10.1172/jci115027
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发表时间：
1991-02-01
影响因子：
15.9
通讯作者：
TABUNI, A
中科院分区：
文献类型：
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作者：
LEVITZ, SM;TABUNI, A
我们研究了人培养巨噬细胞上的受体(MO-M-Phi)与被包裹的和同基因突变的新生隐球菌囊状菌株结合的责任，以及这种结合是否导致吞噬事件。这两个菌株都需要与正常人血清中的补体成分进行调理才能发生结合。结合无囊化的菌株，但不结合被包裹的菌株，导致吞噬。针对MO-M-Pha上的三种已确定的补体受体(CR)中的任何一种(CR1、CR3和CR4)的单抗可深刻地抑制血清调理的包囊化(以及较小程度的无囊化)生物体与MO-M-Phi的结合。免疫荧光研究表明，CR迁移到隐球菌结合部位。胰酶和弹性蛋白酶抑制微囊化酵母和微囊化酵母与MO-M-Phi的结合。被包裹的新生葡萄球菌的结合被在低温中孵育或被受体封顶和肌动蛋白微丝的抑制剂深深地抑制。因此，多个CR似乎有助于MO-M-Phi与血清调理包裹的新生葡萄球菌的结合。结合是一个能量依赖的过程，需要肌动蛋白的构象变化，但不会导致有机体的吞噬作用。相比之下，MO-M-Phi与无囊化酵母的结合不需要能量，结合可以触发吞噬作用。
We studied the receptors on human cultured macrophages (MO-M-phi) responsibility for binding encapsulated and isogenic mutant acapsular strains of Cryptococcus neoformans, and whether such binding leads to a phagocytic event. Both strains required opsonization with complement components in normal human serum in order for binding to occur. Binding of the acapsular, but not the encapsulated, strain led to phagocytosis. MAb directed against any of the three defined complement receptors (CR) on MO-M-pha (CR1, CR3, and CR4) profoundly inhibited binding of serum-opsonized encapsulated (and to a lesser extent acapsular) organisms to MO-M-phi). Immunofluorescence studies demonstrated migration of CR to the area of the cryptococcal binding site. Trypsin and elastase inhibited binding of encapsulated and, to a lesser extent, acapsular yeasts to MO-M-phi. Binding of encapsulated C. neoformans was profoundly inhibited by incubation in the cold or by inhibitors of receptor capping and actin microfilaments. Thus, multiple CR appear to contribute to binding of serum-opsonized encapsulated C. neoformans by MO-M-phi. Binding is an energy-dependent process that requires conformational changes in actin yet does not lead to phagocytosis of the organism. In contrast, energy is not required for binding of acapsular yeasts by MO-M-phi and binding triggers phagocytosis.