Immunohistochemical study of p53 in human lung carcinomas.
Immunohistochemical study of p53 in human lung carcinomas.
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人肺癌中 p53 的免疫组织化学研究。
DOI：
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发表时间：
1993
影响因子：
6
通讯作者：
Christian Brambillat
中科院分区：
文献类型：
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作者：
Elizabeth Brambilla;S. Gazzeri;Denis Moro;C. D. Fromentel;Valerie Gouyer;Michele Jacrot;Christian Brambillat
p53蛋白的免疫组织化学分析进行了95个肺癌的所有组织学类型，包括60个原发性肿瘤，35个淋巴结转移，和36个相应的裸鼠异种移植瘤，使用四种抗体：PAb 240特定的一些突变体的构象，PAb 421，PAb 1801，和CM 1与大多数形式的p53反应。用这四种抗体中的至少两种进行核染色，发现在50%的所有组织学类型的原发性肿瘤（类癌除外）中存在累积的蛋白质，这被认为是p53基因错义突变的指示。通过北方印迹分析在另外26%的肿瘤中检测到信使RNA表达的一些缺陷。p53免疫表型在裸鼠体内基本保持。p53积累与生存率无关，但与疾病的扩展有关（P = 0.01）。最后，免疫组化分析允许识别p53突变的免疫表型在41%的肿瘤中，其中p53 DNA和信使RNA明显正常，使用标准的分子生物学。因此，该方法提供了一种快速有效的方法来研究p53突变导致肺肿瘤细胞中蛋白质的积累。
Immunohistochemical analysis of p53 protein was carried out on 95 lung carcinomas from all histological types, including 60 primary tumors, 35 lymph node metastases, and 36 corresponding nude mice xenografts, using four antibodies: PAb240 specific for some mutant conformations; PAb421, PAb1801, and CM1 reactive with most of the forms of p53. Nuclear staining with at least two of those four antibodies revealed the presence of an accumulated protein, considered as indicative of a missense mutation in the p53 gene, in 50% of primary tumors of all histological types, except carcinoids. Some defect of messenger RNA expression was detected by Northern blot analysis in an additional 26% of tumors. p53 immunophenotype of the original tumor was fairly maintained on nude mice. p53 accumulation was not correlated with survival, but with disease extension (P = 0.01). Finally, immunohistochemical analysis allowed the recognition of p53 mutant immunophenotype in 41% of tumors where p53 DNA and messenger RNA were apparently normal, using standard molecular biology. Thus, this method provides a rapid and efficient approach for studying p53 mutations leading to an accumulated protein in lung tumors cells.