Translocation and association of ROCK-II with RhoA and HSP27 during contraction of rabbit colon smooth muscle cells.
Translocation and association of ROCK-II with RhoA and HSP27 during contraction of rabbit colon smooth muscle cells.
复制标题
兔结肠平滑肌细胞收缩过程中 ROCK-II 的易位和与 RhoA 和 HSP27 的关联。
DOI：
10.1016/j.bbrc.2004.04.159
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Bitar,KhalilN
中科院分区：
文献类型：
--
作者：
Patil,SureshB;Tsunoda,Yasuhiro;Pawar,MercyD;Bitar,KhalilN
本文的重点是了解HSP27介导RhoA与ROCK-II在兔结肠平滑肌细胞持续收缩中的作用。在圆形平滑肌细胞中；乙酰胆碱诱导的收缩（10−7M）与ROCK-II向颗粒部分的转运有关，刺激后4min仍持续（增加135.1±8.1%,P < 0.05）。ROCK-II与RhoA颗粒分数（147.46±9.31,148.22±9.41,n=3, P < 0.05）和HSP27（155.6±10.7%,P < 0.05）的相关性也有所增加。用Y27632对细胞进行预孵育，可抑制颗粒部分中ROCK-II与RhoA的关联。乙酰胆碱（10−7M）诱导MLC持续磷酸化（122.75±9.97%,P < 0.05和174.65±28.36%，分别在30s和4min增加P < 0.05），经Y27632预孵育抑制。结果表明，ROCK-II与RhoA和HSP27一起易位到颗粒部分，表明ROCK-II与RhoA的易位和关联是由HSP27介导的。在HSP27介导的颗粒部分中，维持RhoA与ROCK-II的功能关联似乎对于保持MLC处于磷酸化状态并因此持续收缩是重要的。
The focus of the paper is to understand the role of HSP27 in mediating the association of RhoA with ROCK-II in sustained contraction of smooth muscle cells from the rabbit colon. In circular smooth muscle cells; acetylcholine-induced contraction (10−7M) was associated with translocation of ROCK-II to the particulate fraction, which remained sustained at 4min after stimulation (135.1±8.1% increase, P⩽0.05). There was also an increased association of ROCK-II with RhoA particulate fraction (147.46±9.31 and 148.22±9.41, n=3, P⩽0.05) and with HSP27 (155.6±10.7% increase, P⩽0.05) in the particulate fraction. Pre-incubation of cells with Y27632 resulted in the inhibition of the association of ROCK-II with RhoA in the particulate fraction. Acetylcholine (10−7M) induced sustained phosphorylation of MLC (122.75±9.97%, P⩽0.05 and 174.65±28.36%, P⩽0.05 increase in the di phospho-MLC at 30s and 4min, respectively), which was inhibited upon pre-incubation with Y27632. Results suggest that ROCK-II undergoes a translocation to the particulate fraction with RhoA and with HSP27, suggesting that translocation and association of ROCK-II with RhoA is mediated by HSP27. Maintenance of the functional association of RhoA with ROCK-II in the particulate fraction mediated by HSP27 appears to be important to retain MLC in the phosphorylated state and hence the sustained contraction.
登录
查看更多内容
影响因子：
3.7
作者：
Taggart, MJ;Lee, YH;Morgan, KG
通讯作者：
Morgan, KG
影响因子：
64.8
作者：
Uehata, M;Ishizaki, T;Narumiya, S
通讯作者：
Narumiya, S
Hsp27 是响应铃蟾肽而持续平滑肌收缩的介质。
DOI：
10.1016/0006-291x(91)92065-r
发表时间：
1991
影响因子：
3.1
作者：
Bitar,KN;Kaminski,MS;Hailat,N;Cease,KB;Strahler,JR
通讯作者：
Strahler,JR
DOI：
10.1016/s0021-9258(19)36636-0
发表时间：
1992-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ming C. Gong;Annette FuglsangSlI;Dario Alessill;Sei KobayashiS;Philip Cohenll;Avril V. SomlyoS;A. Somlyo
通讯作者：
Ming C. Gong;Annette FuglsangSlI;Dario Alessill;Sei KobayashiS;Philip Cohenll;Avril V. SomlyoS;A. Somlyo
影响因子：
56.9
作者：
Kimura, K;Ito, M;Kaibuchi, K
通讯作者：
Kaibuchi, K