Magnetoliposomes based on manganese ferrite nanoparticles as nanocarriers for antitumor drugs
Magnetoliposomes based on manganese ferrite nanoparticles as nanocarriers for antitumor drugs
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DOI：
10.1039/c5ra27058h
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发表时间：
2016-02
期刊：
影响因子：
3.9
通讯作者：
A. R. O. Rodrigues;J. Ramos;I. T. Gomes;B. Almeida;J. Araújo;M. Queiroz;P. Coutinho;E. M. Castanheira
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文献类型：
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作者：
A. R. O. Rodrigues;J. Ramos;I. T. Gomes;B. Almeida;J. Araújo;M. Queiroz;P. Coutinho;E. M. Castanheira
采用共沉淀法合成了粒径分布为26 ± 7 nm的锰铁氧体纳米颗粒（TEM测量）。所获得的纳米粒子表现出超顺磁性行为，在室温下的磁平方比为0.016和6.3 Oe。这些纳米粒子被截留在脂质体（水性磁性脂质体，AML）或覆盖有脂质双层，形成固体磁性脂质体（SMLs）。两种类型的磁性脂质体，表现出低于或约150 nm的尺寸，被发现是适合于生物医学应用。磁性脂质体（AMLS和SML）和GUV（巨单层囊泡）（后者用作细胞膜的模型）之间的膜融合通过Forster共振能量转移（FRET）测定来证实，使用NBD标记的脂质作为能量供体，尼罗红或罗丹明B-DOPE作为能量受体。一个潜在的抗肿瘤噻吩并吡啶衍生物被成功地纳入水和固体磁脂质体，指出这些系统在肿瘤治疗中的应用前景，同时作为热疗剂和抗肿瘤药物的纳米载体。
Manganese ferrite nanoparticles with a size distribution of 26 ± 7 nm (from TEM measurements) were synthesized by the coprecipitation method. The obtained nanoparticles exhibit a superparamagnetic behaviour at room temperature with a magnetic squareness of 0.016 and a coercivity field of 6.3 Oe. These nanoparticles were either entrapped in liposomes (aqueous magnetoliposomes, AMLs) or covered with a lipid bilayer, forming solid magnetoliposomes (SMLs). Both types of magnetoliposomes, exhibiting sizes below or around 150 nm, were found to be suitable for biomedical applications. Membrane fusion between magnetoliposomes (both AMLS and SMLs) and GUVs (giant unilamellar vesicles), the latter used as models of cell membranes, was confirmed by Forster Resonance Energy Transfer (FRET) assays, using a NBD labeled lipid as the energy donor and Nile Red or rhodamine B-DOPE as the energy acceptor. A potential antitumor thienopyridine derivative was successfully incorporated into both aqueous and solid magnetoliposomes, pointing to a promising application of these systems in oncological therapy, simultaneously as hyperthermia agents and nanocarriers for antitumor drugs.