REGULATION OF COMPETENCE-SPECIFIC GENE-EXPRESSION BY MEC-MEDIATED PROTEIN-PROTEIN INTERACTION IN BACILLUS-SUBTILIS
REGULATION OF COMPETENCE-SPECIFIC GENE-EXPRESSION BY MEC-MEDIATED PROTEIN-PROTEIN INTERACTION IN BACILLUS-SUBTILIS
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DOI：
10.1073/pnas.91.13.5793
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发表时间：
1994-06-21
影响因子：
11.1
通讯作者：
DUBNAU, D
中科院分区：
文献类型：
--
作者：
KONG, LY;DUBNAU, D
感受态基因在枯草芽孢杆菌中的表达受信号转导级联控制，该信号转导级联在从指数生长到稳定期的过渡期间增加感受态转录因子（CTF，由comK编码）的表达。CTF（ComK）的转录被mecA基因的产物降低，并且这种抑制响应于从调节途径的上游接收的未知信号而被解除。mecA或另一个基因mecB的失活导致ComK的过量产生。我们在这里表明，MecA蛋白的浓度不随培养基的生长阶段的函数，或在主管和非主管细胞显着变化。我们还表明，MecA可以直接与ComK相互作用。最后，证据表明，MecB功能之前，MecA的信号通路。一个模型进行了讨论，其中涉及螯合ComK的MecA结合和释放的转录因子时，适当的信号被中继到MecA的MecB。
The expression of competence genes in Bacillus subtilis is controlled by a signal transduction cascade which increases the expression of a competence transcription factor (CTF, encoded by comK) during the transition from exponential growth to stationary phase. The transcription of CTF (ComK) is decreased by the product of the mecA gene, and this inhibition is relieved in response to an unknown signal received from upstream in the regulatory pathway. Inactivation of either mecA or another gene, mecB, results in overproduction of ComK. We show here that the concentration of MecA protein does not vary markedly with culture medium, as a function of growth stage, or in competent and noncompetent cells. We also show that MecA can interact directly with ComK. Finally, evidence is presented suggesting that MecB functions prior to MecA in the signaling pathway. A model is discussed which involves the sequestration of ComK by MecA binding and the release of the transcription factor when an appropriate signal is relayed to MecA by MecB.