Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression.
Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression.
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长链非编码RNA uc.345通过上调hnRNPL表达促进胰腺癌的肿瘤发生
DOI：
10.18632/oncotarget.12253
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发表时间：
2016-11-01
期刊：
影响因子：
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通讯作者：
Chen H
中科院分区：
文献类型：
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作者：
Liu C;Wang J;Yuan X;Qian W;Zhang B;Shi M;Xie J;Shen B;Xu H;Hou Z;Chen H
越来越多的证据表明，长链非编码RNA在细胞过程中起着重要的功能或调节作用，并且由于lncRNA的异常表达而导致癌症疾病。LncRNA通过与蛋白质的相互作用参与肿瘤的发生发展，发挥重要作用。在本研究中，我们报告了一种名为uc.345的lncRNA，与相应的非癌组织相比，该lncRNA在肿瘤组织中上调。我们发现，较高的uc.345表达水平更频繁地观察到在组织中的浸润深度增加和先进的TNM肿瘤淋巴结转移T阶段。此外，uc.345可作为胰腺癌患者总生存期（OS）的独立危险因素。通过采用软琼脂试验和肿瘤异种移植模型，我们表明uc.345可以加速肿瘤生长。此外，我们发现uc.345可以上调hnRNPL的表达，并且抑制（hnRNPL）可以抑制uc.345的肿瘤发生能力。总的来说，这些结果表明uc.345作为致癌lncRNA发挥作用，促进肿瘤进展，并作为胰腺癌患者总生存期的不良预测因子。
Increasing evidence points to an important functional or regulatory role of long noncoding RNA in cellular processes as well as cancer diseases resulted from the aberrant lncRNA expression. LncRNA could participate in the cancer progression and develop a significant role through the interaction with proteins. In the present study, we report a lncRNA termed uc.345 that is up-regulated in tumor tissues, compared to the corresponding noncancerous tissues. We found that a higher uc.345 expression level was more frequently observed in tissues with increased depth of invasion and advanced TNM tumor node metastasis T stage. Moreover, uc.345 could be used as an independent risk factor for the overall survival (OS) of the pancreatic cancer patients. By employing soft agar assays and tumor xenograft models, we showed that uc.345 could accelerate tumor growth. Further, we discovered that uc.345 could upregulate the hnRNPL expression and that inhibition of (hnRNPL) dampens the tumorigenesis capability of uc.345. Collectively, these results demonstrate that uc.345 functions as an oncogenic lncRNA that promotes tumor progression and serves as a poor predictor for pancreatic cancer patients' overall survival.