Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice
Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice
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DOI：
10.1016/j.intimp.2010.11.019
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发表时间：
2011-02-01
影响因子：
5.6
通讯作者：
Suzuki, Tatsuo
中科院分区：
文献类型：
--
作者：
Kobayashi, Noritada;Saito, Takao;Suzuki, Tatsuo
针对流感病毒（IFV）感染的宿主防御机制涉及先天性和获得性免疫。在其他成分中，气道粘膜中的分泌型免疫球蛋白A（SIgA）在预防IFV感染中起着特别关键的作用。在150株乳酸菌中，戊糖乳杆菌菌株b240（b240）在小鼠派尔集合淋巴结细胞中具有最高的IgA诱导效力。我们以前报告了一个实际的新发现，口服非活热杀死的b240提高人类唾液伊加分泌。在致死水平的IFV A/PR 8/34（H1N1）感染的BALB/c小鼠模型中，在IFV感染前经口灌胃给予b240 3周，可显著延长小鼠的存活期。对于非致死水平的IFV感染，感染后7天肺中IFV的感染性滴度在类似的b240给药后显著降低。第7天，b240治疗组支气管肺泡灌洗液和血浆中的抗IFV伊加和免疫球蛋白C滴度均显著高于对照组。在与b240致敏的脾细胞的混合淋巴细胞反应中，IFV驱动的T细胞因子的产生增加，初步证实了b240应用增强抗IFV免疫应答，这些结果表明，口服非活性热灭活的b240摄入可以促进对IFV感染的保护。(C)2010 Elsevier B. V.保留所有权利。
Host-defense mechanisms against influenza virus (IFV) infection involve both innate and acquired immunities. Among other components, secretory immunoglobulin A (SIgA) in the airway mucosa plays a particularly pivotal role in preventing IFV infection. Among 150 strains of lactic acid bacteria, Lactobacillus pentosus strain b240 (b240) has the highest IgA-inducing potency in mouse Peyer's patch cells. We previously reported a practical new finding that oral ingestion of nonviable heat-killed b240 elevates salivary IgA secretion in humans. The present study aimed to determine if nonviable b240 can prevent IFV infection in mice.In a BALB/c mouse model infected with lethal levels of IFV A/PR8/34 (H1N1), oral administration of b240 for 3 weeks by gavage prior to IFV infection significantly prolonged the survival period. For IFV infection at nonlethal levels, the infectious titers of IFV in the lungs 7 days after infection were significantly reduced after similar b240 administration. Both anti-IFV IgA and immunoglobulin C titers in bronchoalveolar lavage fluid and plasma on day 7 were significantly higher in the b240-treated group than the control group. The augmentation of the anti-IFV immune response by b240 application was preliminarily confirmed by the elevated production of IFV-driven T-cell factors during mixed lymphocyte reactions with b240-primed splenocytes.These results suggest that oral nonviable heat-killed b240 intake can facilitate protection against IFV infection. (C) 2010 Elsevier B.V. All rights reserved.