Multilineage Differentiation Potential of Fibroblast-like Stromal Cells Derived from Human Skin
Multilineage Differentiation Potential of Fibroblast-like Stromal Cells Derived from Human Skin
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DOI：
10.1089/ten.tea.2009.0431
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发表时间：
2010-05-01
影响因子：
4.1
通讯作者：
Chan, Shu-Hui
中科院分区：
文献类型：
--
作者：
Huang, Hsing-, I;Chen, Shao-Kuan;Chan, Shu-Hui
成体干细胞存在于成体组织中，由于其具有自我更新和分化的能力，被认为具有巨大的临床应用潜力。但存在感染风险大、分离率低等局限性，使得寻找合适来源的工作仍需继续。在这里，我们展示了从人类包皮组织样本中分离出祖细胞，这些组织具有成纤维细胞样的形态，可以很容易地繁殖超过50代。这些包皮成纤维样间充质干细胞表达CD90、CD105、CD166、CD73、SH3和SH4，与人骨髓间充质干细胞的免疫表型相似。与人成纤维细胞系Hs68相比，FDSCs表达CD105，不表达CD54。此外，FDSCs可被诱导分化为骨细胞、脂肪细胞、神经细胞、平滑肌细胞、雪旺细胞和肝细胞样细胞。有趣的是，当在Dulbecco的改良Eagle‘s培养基/F12培养时，FDSCs可以形成表达纤维连接蛋白和波形蛋白水平增加的球状细胞。总之，包皮可以作为一种有价值的多能细胞来源，具有内胚层、中胚层和外胚层细胞的能力。这些包皮来源的基质细胞具有易于获取、分离和繁殖的优点，在未来干细胞分化和治疗应用的研究中可能具有潜在的应用价值。
Adult stem cells that reside in adult tissues have been deemed to possess great potential for clinical application because of their capabilities of self-renewal and differentiation. However, the limitations such as infection risks and low isolation rate make the search for appropriate source to be continued. Here, we demonstrate isolation of progenitors from human foreskin tissue samples, which have fibroblast-like morphology and could be easily propagated for more than 50 passages. These foreskin-derived fibroblast-like stromal cells (FDSCs) expressed CD90, CD105, CD166, CD73, SH3, and SH4, which is similar to the immunophenotypes of human bone marrow-derived mesenchymal stem cells. In comparison with Hs68, the human fibroblast cell line, FDSCs are positive for CD105 and absent for CD54 expression. Further, FDSCs could be induced to differentiate into osteocytes, adipocytes, neural cells, smooth muscle cells, Schwann-like cells, and hepatocyte-like cells. Interestingly, when cultured in Dulbecco's modified Eagle's medium/F12 medium, FDSCs can form spheres with increased expression levels of fibronectin and vimentin. In conclusion, foreskin can serve as a valuable source of multipotent cells with the capabilities for endodermal, mesodermal, and ectodermal cells. Coupled with the advantages of their easy access, isolation, and propagation, these foreskin-derived stromal cells might be of potential use in future studies in stem cell differentiation and therapeutic application.