Estimating the chance of success of archaeometric analyses of bone: UV-induced bone fluorescence compared to histological screening
Estimating the chance of success of archaeometric analyses of bone: UV-induced bone fluorescence compared to histological screening
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DOI：
10.1016/j.palaeo.2011.03.021
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发表时间：
2011-09-15
影响因子：
3
通讯作者：
Harbeck, Michaela
中科院分区：
文献类型：
--
作者：
Hoke, Nadja;Burger, Joachim;Harbeck, Michaela
对于大多数考古骨骼材料的考古测量分析，如确定同位素组成或成因方法，成岩改造的高级程度可能使指定分析变得不可能。由于缺乏阳性信号通常是在耗时和成本密集的样品处理后才能看到的，因此显然需要一种易于应用的筛选方法，允许预先选择包含保存完好的生物分子的样品。在这项研究中，我们直观地测定了76个马骨切片的紫外光诱导的自发荧光，所有这些标本都来自不同环境和年代背景的史前考古遗址。为了评估这种方法的筛选潜力，将每个样本的宏观荧光外观与其组织学保存程度进行了比较，这一特征也通常被用作骨中整体生物分子保存的标志。根据紫外线荧光和组织学筛查的阳性/阴性预测价值，测定胶原含量和质量以及DNA分析的聚合酶链式反应成功。其目的是创造一种筛选方法，不仅适用于日常实验室实践，也适用于无法获得精密机器的考古学家，他们需要预先选择最有希望的样本发送给承包商进行考古测量分析。(C)2011爱思唯尔B.V.保留所有权利。
For most archaeometric analyses on archaeological bone material, such as the determination of the isotopic composition or genetic approaches, an advanced degree of diagenetic alteration can make designated analysis impossible. Since the lack of a positive signal is mostly seen only after time consuming and cost intensive sample processing, the need for an easy-to-apply screening method that allows a pre-selection of samples containing well-preserved biomolecules is obvious.In this study, we visually determined the UV-induced autofluorescence of 76 horse bone cross-sections, all from prehistoric archaeological sites of varying environmental and chronological background. In order to assess the screening potential of this method, the macroscopic fluorescence appearance of each sample was compared to its degree of histological preservation, a feature which is also commonly utilised as a marker for overall biomolecular preservation in bone. Collagen content and quality as well as PCR-success for DNA analysis were determined and evaluated with regard to the positive/negative predictive value of UV fluorescence and histological screening. The aim was to create a screening method designed not only for daily laboratory practice, but also for archaeologists with no access to elaborate machinery and who need to pre-select the most promising samples to send out to a contractor for archaeometric analyses. (C) 2011 Elsevier B.V. All rights reserved.