Histopathology of melanosis coli and determination of its associated genes by comparative analysis of expression microarrays.
Histopathology of melanosis coli and determination of its associated genes by comparative analysis of expression microarrays.
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DOI：
10.3892/mmr.2015.4126
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发表时间：
2015-10
影响因子：
3.4
通讯作者：
Li XH
中科院分区：
文献类型：
--
作者：
Li XΑ;Zhou Y;Zhou SΧ;Liu HR;Xu JM;Gao L;Yu XJ;Li XH
结肠黑变病（MC）是指结肠粘膜上异常的棕色或黑色色素沉着。然而，与结肠黑变病发病机制相关的组织病理学发现和基因仍有待充分阐明。本研究旨在探讨MC的组织病理学特征和差异表达基因。这涉及对从诊断为MC的患者分离的组织切片进行苏木精和伊红染色、特异性染色和免疫组织化学。应用基因表达谱芯片、免疫印迹和免疫荧光技术分析大肠黑变病菌的差异表达基因。结果表明，MC内色素沉积物主要由脂褐素组成。TUNEL法显示，大量凋亡细胞存在于巨噬细胞和结肠上皮的浅表固有层内。表达谱芯片分析显示，在大肠黑变病中表达显著下调的基因为CYP3A4、CYP3A7、UGT 2B11和UGT 2B15。Western blotting和免疫荧光检测表明，正常组织中CYP3A4的表达高于MC组织。本研究的结果提供了一个全面的描述MC的组织病理学特征和发病机制，并为第一次，据我们所知，证明细胞色素P450相关基因显着下调结肠黑变病。这一新的信息可用于协助进一步调查结肠黑变病。
Melanosis coli (MC) refers to the condition characterized by abnormal brown or black pigmentation deposits on the colonic mucosa. However, the histopathological findings and genes associated with the pathogenesis of melanosis coli remain to be fully elucidated. The present study aimed to examine the histopathological features and differentially expressed genes of MC. This involved performing hematoxylin and eosin staining, specific staining and immunohistochemistry on tissues sections, which were isolated from patients diagnosed with MC. DNA expression microarray analysis, western blotting and immunofluorescence assays were performed to analyze the differentially expressed genes of melanosis coli. The results demonstrated that the pigment deposits in MC consisted of lipofuscin. A TUNEL assay revealed that a substantial number of apoptotic cells were present within the macrophages and superficial lamina propria of the colonic epithelium. Expression microarray analysis revealed that the significantly downregulated genes were CYP3A4, CYP3A7, UGT2B11 and UGT2B15 in melanosis coli. Western blotting and immunofluorescence assays indicated that the expression of CYP3A4 in the normal tissue was higher than in the MC tissue. The results of the present study provided a comprehensive description of the histopathological characteristics and pathogenesis of MC and for the first time, to the best of our knowledge, demonstrated that the cytochrome P450-associated genes were significantly downregulated in melanosis coli. This novel information can be used to assist in further investigations of melanosis coli.