High-sensitivity and label-free identification of a transgenic genome using a terahertz meta-biosensor
High-sensitivity and label-free identification of a transgenic genome using a terahertz meta-biosensor
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DOI：
10.1364/oe.26.031589
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发表时间：
2018-11-26
期刊：
影响因子：
3.8
通讯作者：
Zhang, Weili
中科院分区：
文献类型：
--
作者：
Yang, Yuping;Xu, Dongqian;Zhang, Weili
在植物生物技术中，高度期望用于鉴定转基因植物基因组的高灵敏度和无标记方法。在这里，我们提出了一个太赫兹（THz）超材料（MM）为基础的生物传感器，包括一个平面阵列的金开口环（SR）谐振器能够感测的谐振频率相当大的转变，由于MM芯片上的介电环境的变化。利用该传感器对转基因番茄基因组DNA进行了太赫兹时域光谱检测。通过改变DNA覆盖层的厚度和介电常数，通过有限元建模证实了实验结果。因此，实现了野生型和转基因组DNA之间的高效和无标记区分。(C)根据OSA开放获取出版协议的条款，2018年美国光学学会
A high-sensitivity and label-free method for identifying a transgenic plant genome is highly desirable in plant biotechnology. Here, we present a terahertz (THz) metamaterial (MM)-based biosensor that comprises a planar array of gold split-ring (SR) resonators capable of sensing a considerable shift in the resonance frequency due to the change of the dielectric environment on the MM chip. The meta-sensor is employed to detect transgenic tomato genome DNAs by THz time-domain spectroscopy. The experimental results were confirmed by finite-element modeling through varying the thickness and dielectric constant of the DNA overlayer. Consequently, high-efficiency and label-free discrimination between the wild-type and transgenic genome DNA was achieved. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement