Ovulation and ovum maturation in the rabbit ovary perfused in vitro.
Ovulation and ovum maturation in the rabbit ovary perfused in vitro.
复制标题
体外灌注兔卵巢的排卵和卵子成熟。
DOI：
10.1095/biolreprod24.3.483
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发表时间：
1981
影响因子：
3.6
通讯作者：
Wallach,EE
中科院分区：
文献类型：
--
作者：
Kobayashi,Y;Wright,KH;Santulli,R;Wallach,EE
体外灌注兔卵巢可作为研究排卵的有用工具。本研究旨在评估0、5、50和100 IU的hCG对排卵效率、hCG给药与卵泡破裂之间的时间间隔、已排卵和未排卵卵子的成熟阶段、卵子成熟度与排卵时间之间的关系的影响，以及原位和体外灌注卵巢中的卵巢水肿。无促性腺激素刺激的卵巢不排卵。排卵效率，即hCG处理后12小时成熟卵泡破裂的百分比，在50或100 IU hCG后相似（分别为78.7%和77.9%），并且在同一动物的右侧和左侧卵巢中没有差异。5IU hCG剂量组排卵率显著降低（P<0.005）。50 IU hCG组排卵时间为6.34 ± 0.14h，100 IU hCG组为5.90 ± 0.12h（P<0.02）。未破裂卵泡释放的卵子的显微镜检查显示卵子处于所有成熟阶段;然而，hCG给药和卵泡破裂之间的时间间隔越短，卵子越不成熟。只有hCG给药后8h或更长时间释放的卵子才达到中期II。未破裂成熟卵泡吸出的卵子变性率高于已排卵卵泡吸出的卵子（P<0.0001）。干重和湿重的比较表明，在体外系统中观察到的卵巢水肿与促性腺激素刺激有关，尽管灌注卵巢比原位卵巢更水肿。这些观察结果表明，卵泡破裂和卵子成熟可以独立进行，并进一步表明，在体外灌注兔卵巢制备可以成功地用于研究排卵的机制。
The in vitro-perfused rabbit ovary serves as a useful tool for investigating ovulation. The present study was designed to evaluate the effects of 0, 5, 50, and 100 IU of hCG on ovulatory efficiency, the time interval between hCG administration and follicle rupture, the stage of maturity of ovulated and unovulated ova, the relationship between ovum maturity and time of ovulation, and ovarian edema in both in situ- and in vitro-perfused ovaries. Ovulation did not occur in ovaries without gonadotropin stimulation. Ovulatory efficiency, the percentage of mature follicles that proceed to rupture by 12 h after hCG treatment, was similar after either 50 or 100 IU of hCG (78.7% and 77.9%, respectively) and did not differ in right and left ovaries of the same animal. A dose level of 5 lU of hCG resulted in a significant reduction (P<0.005) in ovulatory efficiency. Ovulation occurred at 6.34 ± 0.14 h after 50 IU of hCG, and 5.90 ± 0.12 h after 100 IU of hCG (P<0.02). Microscopic examination of ova released from unruptured follicles revealed ova at all stages of maturity; however, the shorter the time interval between administration of hCG and follicle rupture, the less mature the ovum. Only those ova released 8 h or more after hCG administration had attained metaphase II. A greater percentage of ova aspirated from unruptured mature follicles were degenerated when compared with ovulated ova (P<0.0001). Comparisons of dry weight and wet weight suggest that ovarian edema observed in the in vitro system is associated with gonadotropin stimulation although perfused ovaries are more edematous than in situ ovaries. These observations suggest that follicle rupture and ovum maturation can proceed independently of one another and further indicate that the in vitro-perfused rabbit ovary preparation can be successfully used to study the mechanism of ovulation.