Molecular characterization of stress-inducible GmNAC genes in soybean
Molecular characterization of stress-inducible GmNAC genes in soybean
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DOI：
10.1007/s00438-009-0436-8
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发表时间：
2009-03
影响因子：
3.1
通讯作者：
L. Tran;Truyen N. Quach;Satish K. Guttikonda;Donavan L. Aldrich;Rajesh Kumar;A. Neelakandan;B. Valliyodan-B.-Va
中科院分区：
文献类型：
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作者：
L. Tran;Truyen N. Quach;Satish K. Guttikonda;Donavan L. Aldrich;Rajesh Kumar;A. Neelakandan;B. Valliyodan-B.-Va
干旱对植物生长和发育是有害的，并且经常导致重要经济作物如大豆（Glycine maxL.）的产量的显著损失。NAC转录因子是一类植物特异性转录因子家族，具有增强植物耐旱性的作用。本研究从大豆中鉴定并克隆了31个含有完整的GmNAC开放阅读框架的unigene。利用酵母单杂交系统对31个GmNAC蛋白的C端调控结构域进行分析，发现其中28个具有转录激活活性。对这些GmNAC基因的表达分析表明，它们在不同器官中的表达存在差异，表明它们在植物生长发育过程中具有不同的功能。为了寻找干旱诱导的GmNAC基因，我们通过实时荧光定量PCR分析预筛选并重新证实，9个GmNAC基因在地上部和根部都受到脱水胁迫的诱导，且诱导水平不同。此外，还研究了这9个GmNAC基因在高盐、低温和脱落酸激素处理下的表达谱。与先前报道的干旱诱导NAC蛋白的拟南芥和水稻的GmNAC蛋白的系统发育分析表明，9个干旱诱导的GmNAC蛋白属于“胁迫诱导”NAC组。GmNAC家族的系统分析结果将为改良耐旱转基因大豆品种的开发提供新的工具和资源。
Drought is detrimental to plant growth and development, and often results in significant losses to the yields of economically important crops such as soybeans (Glycine maxL.). NAC transcription factors (TFs), which consist of a large family of plant-specific TFs, have been reported to enhance drought tolerance in a number of plants. In this study, 31 unigenes that contain the complete open reading frames encoding GmNAC proteins were identified and cloned from soybean. Analysis of C-terminal regulatory domain using yeast one-hybrid system indicated that among 31 GmNAC proteins, 28 have transcriptional activation activity. Expression analysis of theseGmNACgenes showed that they are differentially expressed in different organs, suggesting that they have diverse functions during plant growth and development. To search for the drought-inducibleGmNACgenes, we prescreened and re-confirmed by quantitative real-time PCR analysis that nineGmNACgenes are induced by dehydration stress with differential induction levels in both shoot and root. The expression profiles of these nineGmNACgenes were also examined under other stresses such as high salinity, cold and with abscisic acid hormone treatments. Phylogenetic analysis of the GmNAC proteins with previously reported drought-inducible NAC proteins ofArabidopsisand rice revealed that the nine drought-inducible GmNAC proteins belong to the “stress-inducible” NAC group. The results of this systematic analysis of the GmNAC family will provide novel tools and resources for the development of improved drought tolerant transgenic soybean cultivars.